G004682
HPLC Analysis of Sugars and Sugar Enantiomers on Astec® CYCLOBOND I 2000
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)
mobile phase: [A] water; [B] acetonitrile; (15:85, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: ELSD at 35°C and 3.2 bar nitrogen
injection: 10 μL
sample: 0.5 mg/mL each in water:acetonitrile, 25:75
suitability
application for HPLC
application(s)
food and beverages
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
D-(−)-Fructose, ≥99% (HPLC), BioReagent, suitable for cell culture, suitable for insect cell culture
D-(+)-Glucose monohydrate, meets analytical specification of Ph. Eur., BP, Ph Franç., 97.5-102.0% anhydrous basis(HPLC)
Sucrose, ≥99.5% (GC)
D-(+)-Maltose monohydrate, ≥99% (HPLC), BioXtra
Lactose, tested according to Ph. Eur.
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