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Merck
CN

G004737

HPLC Analysis of Triazole Antifungal Agent Enantiomers on Astec® CYCLOBOND I 2000 HP-RSP

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Astec CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)
mobile phase: [A] 0.1% formic acid; [B] acetonitrile; (70:30, A:B)
flow rate: 1 mL/min
detector: UV, 230 nm
injection: 1 μL
sample: 0.1 mg/mL in methanol
peaks 1&2: enilconazole
peaks 2&3: eberconazole
peaks 4&5: miconazole
peaks 6&7: ketoconazole

suitability

application for HPLC

application(s)

chemicals and industrial polymers

Analysis Note

Note: Peak 2 of enilconzaole coelutes with peak 1 of eberconazole under these conditions.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

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