technique(s)
HPLC: suitable
test parameters
column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A]: water; [B]: acetonitrile
gradient: held at 25% for 1.5 min; 25 to 85% B in 12 min; held at 85% B for 1 min
flow rate: 1.0 mL/min
pressure: 3481 psi (240 bar)
column temp.: 60 °C
detector: UV, 205 nm
injection: 25 μL
sample: 50 μg/mL in water:methanol (82:18)
suitability
application for HPLC
application(s)
vitamins, nutraceuticals, and natural products
Analysis Note
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of ginseng.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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