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Merck
CN

G005401

HPLC Analysis of Steroids on Ascentis® Express C18 (10 cm x 2.1 mm I.D.)

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] water; [B] acetonitrile; (60:40, A:B)
flow rate: 0.2 mL/min
pressure: 2944 psi (203 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 2 μL
sample: 50 mg/L in 75:25, water:methanol

suitability

application for HPLC

application(s)

clinical

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 5 steroids.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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