G005482
LC/MS Analysis of Lactic Acid Enantiomers in Urine on Astec® CHIROBIOTIC® R
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Astec CHIROBIOTIC R, 15 cm x 2.1 mm I.D., 5 μm particles (13019AST)
mobile phase: [A] 33.3 mM ammonium acetate; [B] acetonitrile; (15:85, A:B)
flow rate: 0.7 mL/min
column temp.: 4 °C
detector: (-)ESI
injection: 20 μL
sample: Urine extracts in 5% (v/v) acetic acid, 10% (v/v) H2O and 85% (v/v) acetonitrile
suitability
application for HPLC
application(s)
clinical
Analysis Note
Chromatogram supplied courtesy of H. Henry and associates at the Clinical Chemistry Laboratory, Centre Hospitalier Universitaire Vaudois, University of Lausanne, CH-1011, Switzerland. View the related full-length contributed article in Reporter 30.1.
Other Notes
Reference: Biomed. Chromatogr. 2011 "Sensitive determination of D-lactic acid and L-lactic acid in urine by high-performance liquid chromatography–tandem mass spectrometry" H. Henry, N. Marmy Conus, P. Steenhout, A. Béguin and O. Boulat, Published online in Wiley Online Library, wileyonlinelibrary.com/journal/bmc.
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
Analyte
Description
L-(+)-Lactic acid, analytical standard
D-(−)-Lactic acid, ≥90%
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service