G005539
HPLC Analysis of Fusarium Mycotoxins on Ascentis® Express Phenyl-Hexyl
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)
mobile phase: [A] water, acetonitrile, 90:10; [B] acetonitrile
gradient: 0% B held for 0.5 min; to 15% B in 1 min; held at 15% B for 6 min
flow rate: 0.3 mL/min
pressure: 192 bar (2780 psi)
column temp.: 30 °C
detector: UV, 218 nm
injection: 10 μL
sample: 5 mg/L ea. in 95:5, water:acetonitrile
suitability
application for HPLC
application(s)
agriculture
food and beverages
Analysis Note
The trichothecene vomitoxins of Fusarium are significant plant pathogens of grain and forage crops that can lead to anorexic effects with livestock. This application demonstrates resolution of deoxynivalenol and three of its analogs.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
Nivalenol hydrate
15-Acetyldeoxynivalenol solution
3-Acetyldeoxynivalenol
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