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Merck
CN

G005689

LC/MS Analysis of Conjugated Bile Acids on Ascentis® Express RP-Amide

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 5 cm x 4.6 mm I.D., 2.7 μm particles (53922-U)
mobile phase: [A] 10 mM ammonium acetate, pH 3 with acetic acid; [B] acetonitrile
gradient: 25 to 40% B in 10 min
flow rate: 1.0 mL/min (postcolumn split to the MS)
column temp.: 35 °C
detector: MS, ESI(-), SIR
injection: 5 μL
sample: 10 mg/L in mobile phase A

suitability

application for HPLC

application(s)

clinical

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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