G005710
LC/MS Analysis of Omeprazole and Metabolites on Ascentis® Express HILIC
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express HILlC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
mobile phase: [A] 5 mM ammonium formate in water, pH 6.5; [B] 5 mM ammonium formate in acetonitrile, pH 6.5; (2:98, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z 50-400
injection: 0.1 μL
sample: 1 mg/L each in mobile phase
suitability
application for HPLC
application(s)
clinical
Analysis Note
This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of omeprazole metabolites.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
Omeprazole sulfone
Omeprazole
5-Hydroxyomeprazole
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