G005712
LC/MS Analysis of Omeprazole and Metabolites on Ascentis® Express RP Amide (pH 3.4)
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 μm particles (53911-U)
mobile phase: [A] 10 mM ammonium formate in water, pH 3.4; [B] 10 mM ammonium formate in methanol, pH 3.4; (65:35, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z 50-400
injection: 1 μL
sample: 1 mg/L in mobile phase
suitability
application for HPLC
application(s)
clinical
Analysis Note
This application demonstrates the suitability of the Ascentis Express RP Amide for the analysis of omeprazole metabolites.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
5-Hydroxyomeprazole
Omeprazole
Omeprazole sulfone
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