G005716
HPLC Analysis of Acetaminophen, Caffeine, Chlorpheniramine, Ibuprofen, Naproxen, Phenylephrine, and Pseudoephedrine on Ascentis® Express C18
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase: [A] 5 mM ammonium phosphate monobasic, pH 2.0 with phosphoric acid: acetonitrile (98:2); [B] acetonitrile;
gradient: 0 to 68% B in 3 min; held at 68% B for 2 min
flow rate: 0.4 mL/min
column temp.: 35 °C
detector: UV, 210 nm
injection: 1 μL
sample: 100 μg/mL in 95:5, water: methanol
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
Analysis Note
This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of various over the counter drugs.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
Maleic acid
Acetaminophen
Caffeine
Naproxen
Ibuprofen
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