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G005740

HPLC Analysis of Spice Cannabinoids on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] 5 mM ammonium formate water; [B] 5 mM ammonium formate (95:5, acetonitrile:water)
gradient: 68 to 100% B in 8 min; held at 100% B for 2 min
flow rate: 0.6 mL/min
pressure: 4351 psi (300 bar)
column temp.: 30 °C
detector: UV, 265 nm
injection: 3 μL

suitability

application for HPLC

application(s)

forensics and toxicology

Analysis Note

Synthetic cannabinoids (e.g. "Spice") are a type of designer drug used to get a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds in the blood or urine suspected users is a significant analytical challenge facing forensic chemists. Here is shown the selectivity provided by C18 phase chemistry on an Ascentis Express C18 column with UV detection. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    JWH-073 N-(3-hydroxybutyl) metabolite solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    Spice Cannabinoid Mix solution, 100 μg/mL each component in acetonitrile, ampule of 1.0 mL, certified reference material, Cerilliant®
    JWH-073 solution, 100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
    HU-210 solution, 100 μg/mL in methanol, ampule of 1 mL, (Spice Cannabinoid), certified reference material, Cerilliant®
    JWH-018 solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

analytical column

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