G005790
LC/MS Analysis of L-Methionine Sulfoximine, Sulfone and Sulfoxide on Ascentis® Express HILIC
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express HILIC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)
mobile phase: [A] acetonitrile; [B] water with 0.1% formic acid, pH 3.5 with ammonium formate; (75:25, A:B)
flow rate: 0.4 mL/min
pressure: 1350 psi 93 bar
temp.: 25 °C
detector: MS, ESI (+), TIC, m/z 150-300
injection: 5 μL
sample: 10 μg/mL in 90:10, methanol:water
suitability
application for HPLC
application(s)
life science and biopharma
Analysis Note
L-methionine sulfoximine and L-buthionine sulfoximine are used in are used to prevent additional enzyme activity in Chinese hampster ovary (CHO) cell lines with supplemental Glutamine Synthase (GS). It is of interest to be able to monitor both purity of such molecules in order to control addition to cell media as well as for the assay of parent molecules during use. The analytes are highly polar and thus should be amenable to HILIC-LC-MS analysis. This first installment investigates the ability of Ascentis Express HILIC and Ascentis Express OH5 stationary phases for the separation of the methionine analog and related compounds as well as methionine and buthionine separations.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
L-Methionine sulfone
L-Methionine sulfoximine, suitable for cell culture
L-Methionine sulfoxide
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