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G005806

LC/MS Analysis of Vitamin D2, D3, Gamma- and Alpha-Tocopherol on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] water; [B] acetonitrile; (5:95, A:B)
flow rate: 0.4 mL/min
column temp.: 40 °C
detector: MS, ESI+
injection: 2 μL
sample: 1 μg/mL in acetonitrile

suitability

application for HPLC

application(s)

clinical
vitamins, nutraceuticals, and natural products

Analysis Note

Analysis was conducted using standard solution, detection on LC/MS system.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Vitamin D2 solution, 100 μg/mL in ethanol, 98% (CP)
    Vitamin D3 solution, 100 μg/mL in ethanol, 97% (CP)
    (+)-γ-Tocopherol, analytical standard
    (±)-α-Tocopherol, analytical standard

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