G005895
LC/MS/MS Analysis of Steroid Hormones in Plasma on Ascentis® Express C18 after Sample Prep using HybridSPE®-Phospholipid
application for HPLC
technique(s)
HPLC: suitable
test parameters
SPE tube/cartridge: HybridSPE®-Phospholipid, 96-well plate, 50 mg/well (575656-U)
sample addition: To each well add 100 μL of plasma followed by 300 μL of precipitation solvent (1% formic acid or 0.5% citric acid in acetonitrile). (Agitate via vortex for 4 minute, place on vacuum manifold and apply 10" Hg vacuum for 4 minutes. Collect filtrate and analyze directly.)
column: Ascentis® Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
gradient: 60% B for 3 min, 60% B to 95% B in 5 min, held at 95% B for 2 min
mobile phase: [A] 5 mM ammonium formate pH 4.0 with formic acid; [B] methanol
flow rate: 0.3 mL/min
column temp.: 50 °C
detector: ESI+, MRM
injection: 2 μL
suitability
application for HPLC
application(s)
clinical
Analysis Note
Legal Information
Analyte
SPE tube or plate
analytical column
standard
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