G005899
HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-300 after Partial Proteolysis with IdeS Protease
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Zenix SEC-300, 30 cm X 4.6 mm I.D., 3 μm particles (Z777087)
mobile phase: 0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate: 0.35 mL/min
pressure: 1350 psi (93 bar)
column temp.: ambient
detector: UV, 215 nm
injection: 1 μL
sample: digested SO57 mAb, ~0.5 g/L
suitability
application for HPLC
application(s)
life science and biopharma
Analysis Note
Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc from the intact IgG, after partial digestion with IdeS protease.
Legal Information
Zenix is a registered trademark of Sepax Technologies
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