G006147
UHPLC Analysis of Corticosteroids on Titan C18
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)
mobile phase: [A] water; [B] acetonitrile; (70:30, A:B)
gradient: 30% to 60% B in 3 minutes, hold for 0.2 min
flow rate: 0.4 mL/min
pressure: 7423 psi (512 bar)
column temp.: 35 °C
detector: UV, 240 nm
injection: 1 μL
sample: 1000 ng/ml in mobile phase
detector: ESI (+) MS TIC SIR
suitability
application for HPLC
application(s)
clinical
pharmaceutical (small molecule)
Analysis Note
Analysis of Corticosteroids on a Titan C18 UPLC column with MS detection in ESI (+) Mode
Other Notes
Other MS Conditions:
- PolarityES+
- Capillary (kV)1.40
- Cone (V)54.00
- RF (V)2.50
- Extractor (V)3.00
- Source Temperature (°C)150
- Desolvation Temperature (°C)500
- Cone Gas Flow (L/Hr)0
- Desolvation Gas Flow (L/Hr)1000
- Collision Gas Flow (mL/Min)ON
- LM 1 Resolution13.64
- HM 1 Resolution13.91
- Ion Energy 10.20
Legal Information
Analyte
Description
Corticosterone, ≥92%
Cortisone, ≥95%
Hydrocortisone, ≥98% (HPLC)
Cortisone 21-acetate, ≥99%
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