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Merck
CN

G006164

HPLC Analysis of Oenothein B on Ascentis® Express RP-Amide, UV and MS Detection

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase: [A] 0.2% formic acid; [B] methanol
gradient: 5% B for 2 min; to 90% B over 18 min; held at 90% B for 10 min
flow rate: 0.6 mL/min
column temp.: 35 °C
detector: UV, 254 nm
detector: MS (ion trap), m/z 100-1500
injection: 2 μL
sample: 1 mg/mL in methanol

suitability

application for HPLC

application(s)

life science and biopharma
pharmaceutical (small molecule)

Analysis Note

Optimization of a published method showing much better retention, peak shape and resolution on Supelco Ascentis Express RP-Amide 2.7 μm compared to a C18 phase. Isomers and small amounts of homologues are resolved and detectable with higher sensitivity.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Oenothein B, analytical standard

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