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Merck
CN

G006189

HPLC Analysis of Phenyl Urea Pesticides on Ascentis® Express C18 Phase

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis® Express C18, 10 cm x 4.6 mm I.D., 2.7 μm particles (53827-U)
mobile phase: [A] 0.025 M potassium phosphate buffer, adj. to pH = 2.5, [B] acetonitrile; (50:50, A:B)
flow rate: 2.0 mL/min
pressure: 300 bar
column temp.: 30 °C
detector: UV 245 nm, VWD
injection: 0.5 μL
sample: acetonitrile

suitability

application for HPLC

application(s)

agriculture
environmental

General description

This separation illustrates the use of the highly efficient 2.7 um Ascentis Express Fused-Core C18 stationary phase in the analysis of some popular herbicides. The short run times allow analyses using isocratic conditions so that column equilibration time is not required between runs.

Other Notes

Response Time: 0.02 sec.
Flow Cell: 2.5 μL semi-micro

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Monuron, PESTANAL®, analytical standard
    Fluometuron, analytical standard
    Isoproturon, PESTANAL®, analytical standard
    Diuron, PESTANAL®, analytical standard
    Linuron, analytical standard
    Neburon, PESTANAL®, analytical standard

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