HPLC Analysis of Column Test Mixture on Ascentis^® Express RP-Amide

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RPA 15 cm x 4.6 mm, 5 μm particles (50773-U)
mobile phase: [A] 20 mM potassium phosphate, pH 7; [B] acetonitrile; (70:30, A:B)
flow rate: 4.0 mL/min
pressure: 4467 psi (308 bar)
column temp.: 26 °C
detector: UV, 254 nm
injection: 5 μL
sample: 1. Uracil (0.016 mg/mL), 2. Benzamide (0.161 mg/mL), 3. Aniline (0.161 mg/mL), 4. Cinnamyl Alcohol (0.016 mg/mL), 5. Dimethyl Phthalate (0.323 mg/mL), (6. 2-Nitroaniline (0.081 mg/mL), 7. 4′-Bromoacetanilide (0.040 mg/mL), 8. 2,2′-Biphenol (0.161 mg/mL), 9. 4,4′-Biphenol (0.040 mg/mL), in 50/50 Water/Acetonitrile)

suitability

application for HPLC

application(s)

general analytical