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Merck
CN

G006340

UHPLC Analysis of Methotrexate and Related Compounds on Ascentis® Express C18

application for UHPLC

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technique(s)

UHPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 μm particles (50813-U)
mobile phase: [A] 10 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (90:10, A:B)
flow rate: 0.7 mL/min
pressure: 1153 psi (795 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 1 μL
sample: 50 μg/mL in 85:15, water:methanol

suitability

application for UHPLC

application(s)

pharmaceutical (small molecule)

Analysis Note

Methotrexate (MTX) has been selected as a tracer of surface contamination exposure of hospital staff to antineoplastic drugs (ANDs). Its analysis is an emerging facet of occupational medicine in hospital premises. Methotrexate and the related compounds aminopterin and folic acid are shown here separated by UHPLC on an Ascentis Express C18 column in less than two minutes. The rapid, high resolution separation means the method can be employed in hospitals or clinical settings to routinely monitor the contamination levels of surfaces.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Aminopterin, powder
    Folic acid, analytical standard
    Methotrexate hydrate, ≥98% (HPLC), powder

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