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Merck
CN

G1006529

HPLC Analysis of Proteins on Proteomix® WCX-NP5

application for HPLC

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technique(s)

HPLC: suitable

test parameters

mobile phase: [A] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [B] 10 mM sodium phosphate, dibasic, 1M sodium chloride, pH 7 with phosphoric acid
gradient: 0 to 60% in 20 min
flow rate: 0.3 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 5 μL
sample: ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23 g/L
column: Proteomix WCX-NP5, 5 cm x 4.6 mm I.D., 5 μm particles (Z777193)
pressure: 420 psi total pressure (29 bar)

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

Four commonly studied proteins were chromatographed using a Proteomics WCX-NP5 column to examine retention time and resolution. It was found that the Proteomics WCX-NP5 column does not resolve the proteins as well as the Proteomics SCX-NP5, strong cation exchange column.

Legal Information

Proteomix is a registered trademark of Sepax Technologies

Analyte

Description
    Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein
    α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder
    Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture
    Lysozyme from chicken egg white, powder or granules, ≥39,000 units/mg protein

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