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Merck
CN

G1006918

TLC Analysis of Cholesterol and its Bile Acid Metabolites on HPTLC Pre-Coated Plate RP-18 F254s

application for TLC

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technique(s)

thin layer chromatography (TLC): suitable

test parameters

plate: TLC pre-coated plate RP-18 F 254s (1.15389.0001,1.15423.0001)
eluent: [A] methanol; [B] acetone; [C] IN acetic acid; (85:10:5)
migration distance: 10 cm
chamber: normal chamber without chamber saturation
sample: 0.4 μL
detector:
dipped in dichlorofluorescein (0.2% in ethanol) (Spray reagent: MnCl2 - sulfuric acid heated up to 120 C for 120 min, UV 366 nm)

suitability

application for TLC

application(s)

life science and biopharma

Analyte

Description
    Cholesterol
    7-Hydroxycholesterol
    Lithocholic acid
    Cholic acid methyl ester
    Cholic acid
    Dehydrocholic acid

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