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Merck
CN

G1007123

UHPLC Analysis of a Rabbit Secretory IgA Monoclonal Antibody on BIOshell A400 Protein C4

application for HPLC

Synonym(s):

immunoglobulin, lgA, peptide, protein, reversed-phase, secretory

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technique(s)

HPLC: suitable

test parameters

column: BIOshell A400 Protein C4; 10 cm x 2.1 mm I.D., 3.4 μm particles (66825-U)
mobile phase: [A] 0.1% TFA in water; [B] 0.1% TFA in acetonitrile
gradient: [A] 0.1% TFA in water; [B] 0.1% TFA in acetonitrile
flow rate: 0.2 mL/min
pressure: 550 psi (38 bar)
column temp.: 75 °C
detector: UV, 215 nm
injection: 1 μL
sample: Rabbit secretory IgA, 100 μg/mL, 0.05% TFA in water

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

Secretory immunoglobulin A (IgA) exists as a complex of 2-4 IgA monomers linked through a 15 kDa polypeptide J chain. Rabbit secretory IgA is a 385 kDa antibody composed of two IgA monomers and the J chain. Secretory IgA is resistant to degradative enzymes; therefore, it plays a key role in providing protection against microbes that multiply in bodily secretions. This antibody was chromatographically analysed on a BIOshell A400 Protein C4 column. A prominent shoulder peak was partially resolved from the main analyte peak, indicating the presence of impurities and/or other, post-translationally modified variants.

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