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Merck
CN

G1007232

LC/MS Analysis of Organophosphonate Nerve Agent Metabolites on SeQuant® ZIC-HILIC

application for LC-MS

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technique(s)

LC/MS: suitable

test parameters

column: SeQuant ZIC-HILIC, PEEK, 150 x 2.1 mm, 5 μm, 200 Å (1.50454.0001)
mobile phase: [A] acetonitrile; [B] ammonium acetate; (80:20, A:B)
flow rate: 0.35 mL/min
detector: ESI-MS in negative ion mode (TIC from SIM m/z: 179 (+ fragment 109, 123, 137, 151, 191, 247 and 286) )
injection: 2 μL samples diluted in 70:30 acetonitrile/water

suitability

application for LC-MS

application(s)

chemicals and industrial polymers

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    PMPA (Pinacolyl-methyl-phosphonic acid)
    BMPA (n-Butyl-methyl-phosphonic acid)
    IEPA (Isopropyl-ethyl-phosphonic acid)
    IMPA (Isopropyl-methyl-phosphonic acid)
    EMPA (Ethyl-methyl-phosphonic acid)
    PPA (Propyl-phosphonic acid)
    EPA (Ethyl-phosphonic acid)
    MPA (Methyl-phosphonic acid)
    See All (8)

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