V000961
Neutral Red
Synonym(s):
3-Amino-7-dimethylamino-2-methylphenazine hydrochloride, Basic Red 5, Toluylene red
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About This Item
Empirical Formula (Hill Notation):
C15H17ClN4
CAS Number:
Molecular Weight:
288.78
PubChem Substance ID:
EC Number:
209-035-8
Beilstein/REAXYS Number:
3918740
MDL number:
SMILES string
Cl.CN(C)c1ccc2nc3cc(C)c(N)cc3nc2c1
InChI
1S/C15H16N4.ClH/c1-9-6-13-15(8-11(9)16)18-14-7-10(19(2)3)4-5-12(14)17-13;/h4-8H,16H2,1-3H3;1H
InChI key
PGSADBUBUOPOJS-UHFFFAOYSA-N
pH
6.8-8.0, red to yellow
mp
290 °C (dec.) (lit.)
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Application
Useful as an indicator for preparing neutral red paper, and as a biological stain.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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[Vital staining of lysosomes and other cell organelles of the rat with neutral red (a summary)].
J Winckler
Acta histochemica. Supplementband, 16, 15-22 (1976-01-01)
V Zuang
Alternatives to laboratory animals : ATLA, 29(5), 575-599 (2001-10-18)
The neutral red release (NRR) assay is a cytotoxicity test that can be used to measure the immediate toxic effects of test substances on the cell membrane, resulting in the leaking of intracellular contents. The assay has already been used
Intracellular pH determination by absorption spectrophotometry of neutral red.
J C LaManna
Metabolic brain disease, 2(3), 167-182 (1987-09-01)
C Svendsen et al.
Ecotoxicology and environmental safety, 57(1), 20-29 (2003-12-09)
The ability of biomarkers to integrate effects of chemicals on biota has lead to increased calls for their application in assessing the status of polluted ecosystems. In tandem there has been an increase in our knowledge of the ecophysiological responses
Maqusood Ahamed et al.
Chemosphere, 135, 278-288 (2015-05-13)
Nickel ferrite nanoparticles (NPs) have received much attention for their potential applications in biomedical fields such as magnetic resonance imaging, drug delivery and cancer hyperthermia. However, little is known about the toxicity of nickel ferrite NPs at the cellular and
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