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Merck
CN

V800090

Bromothymol Blue

Powder

Synonym(s):

3′,3″-Dibromothymolsulfonphthalein

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About This Item

Empirical Formula (Hill Notation):
C27H28Br2O5S
CAS Number:
76-59-5
Molecular Weight:
624.38
UNSPSC Code:
12171502
PubChem Substance ID:
329829148
EC Number:
200-971-2
Beilstein/REAXYS Number:
373934
MDL number:
MFCD00005872

Key Documents

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Product Name

Bromothymol Blue,

InChI

1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3

SMILES string

CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C

InChI key

NUHCTOLBWMJMLX-UHFFFAOYSA-N

product line

Vetec™

form

powder

visual transition interval

5.8-7.6, yellow to blue

mp

200-202 °C (lit.)

λmax

430-435 nm (pH 5.8), 615-618 nm (pH 7.6)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
MMBB4458
MMBB4369

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Hisataka Maruyama et al.
Lab on a chip, 8(2), 346-351 (2008-01-31)
This paper demonstrates local pH measurement in a microchip using a pH-sensing gel-microbead. To achieve this, the gel-microbead made of a hydrophilic photo-crosslinkable resin was functionalized with the pH indicator bromothymol blue (BTB). The primary constituent of this photo-crosslinkable resin
Olga A Zaporozhets et al.
Analytica chimica acta, 597(1), 171-177 (2007-07-31)
Solid-phase spectrophotometric and visual test-methods of fluoride and oxalate determination are proposed. The methods are based on the competitive reactions of ZrOCl2 with methylthymol blue immobilized on silica gel and fluoride or oxalate in solution. Absorbance of the solid-phase reagent
Bambang Kuswandi et al.
Talanta, 74(4), 613-618 (2008-03-29)
An optical fiber biosensor consisting of acetylcholinesterase (AChE) and bromothymol blue (BTB) doped sol-gel film was employed to detect organophosphate pesticide chlorpyrifos. The main advantage of this optical biosensor is the use of a single sol-gel film with immobilized AChE
Sevgi Tatar Ulu
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 67(3-4), 778-783 (2006-10-13)
A simple, rapid, accurate, precise and sensitive colorimetric method for the determination of finasteride in tablets is described. The proposed methods are based on the formation of ion-pair complexes between the examined drug with bromophenol blue (BPB), bromocresol green (BCG)
Mariana A Sanchez et al.
Analytica chimica acta, 694(1-2), 95-99 (2011-05-14)
Liquid-liquid microextraction without phase segmentation was implemented in a multicommuted flow system for determination of the anti-hypertensive diltiazem. The procedure was based on ion pair formation between the drug and the dye bromothymol blue at pH 3.5. The detection was
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