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Merck
CN

V900888

Thiazolyl Blue Tetrazolium Bromide

98%, reagent grade, powder

Synonym(s):

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide

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About This Item

Empirical Formula (Hill Notation):
C18H16BrN5S
CAS Number:
298-93-1
Molecular Weight:
414.32
UNSPSC Code:
12171500
PubChem Substance ID:
329830220
EC Number:
206-069-5
Beilstein/REAXYS Number:
4081397
MDL number:
MFCD00011964

Key Documents

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Product Name

Thiazolyl Blue Tetrazolium Bromide, Vetec™, reagent grade, 98%

InChI key

AZKSAVLVSZKNRD-UHFFFAOYSA-M

InChI

1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1

SMILES string

[Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4

grade

reagent grade

product line

Vetec™

assay

98%

form

powder

mp

195 °C (dec.) (lit.)

solubility

H2O: 5 mg/mL, clear

suitability

suitable for

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

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Application

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
WXBD9203V
WXBD1524V
WXBC6835V
WXBC4390V
WXBC2166V

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