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AOX-15

Zen-Bio

Zen-Bio® Ferrous Iron Chelating (FIC) Assay Kit

Two 96-Well Plates Kit Including EDTA Controls

Synonym(s):

Transition Metal Ions

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General description

Excess transition metal ions, such as Fe(II), can generate hydroxyl radicals [OH•] in biological systems through Fenton-like reactions. Some antioxidants are able to chelate Fe(II) thereby inhibiting the formation of hydroxyl radicals and oxidative damage. The ZenBio Ferrous Ion Chelating (FIC) Assay measures the capacity of test samples to chelate free ferrous ions in solution thereby inhibiting Fe(II) binding to ferrozine which generates a highly colored complex. EDTA serves as a positive control capable of chelating ferrous ions in a dose dependent manner. The FIC assay is an endpoint assay measuring absorbance of the ferrous-ferrozine complex at λ = 562nm. FIC activity is determined as the percent of total ferrozine / Fe(II) binding.


The AOX-15 kit contains everything required for performing the FIC assay using two 96-well plates including EDTA controls.

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