Zen-Bio® ORAC Antioxidant Assay Kit

The ZenBio ORAC (Oxygen Radical Absorbance Capacity) Antioxidant Assay Kit measures the loss of fluorescein fluorescence over time due to peroxyl-radical formation by the breakdown of AAPH (2,2'-azobis-2-methyl-propanimidamide, dihydrochloride). Trolox [6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid], a water soluble vitamin E analog, serves as a positive control inhibiting fluorescein decay in a dose dependent manner. The ORAC assay is a kinetic assay measuring fluroescein decay and antioxidant protection over time. The antioxidant activity in biological fluids, cells, tissues, and natural extracts can be normalized to equivalent Trolox units to quantify the composite antioxidant activity present. This assay measures antioxidant activity by hydrogen atom transfer and when combined with ZenBio's other antioxidant assay kits, can provide a comprehensive analysis of a test sample's antioxidant activity.

The AOX-2RB kit contains reagents sufficient for five 96-well plate test using the inner 60 wells.