containing pTZ
18R strain was plated on LB amp+ plates. The JM 101
(ESC 157) strain was plated on LB amp- plates. The
plates were incubated at 37 °C for 24-48 hours. The ESC
206 containing pTZ 18R
Cat. #64-101
siIMPORTER™ Transfection Reagent
12
2. Using a 1.6ml microcentrifuge tube, dilute the
siIMPORTER™ reagent into serum-free medium
that has been warmed to 37°C according
Technol., 68, 91-101 (2017)
4. Huettner, J. E., and Baughman, R. W., J.
Neurosci., 6(10), 3044-3060 (1986).
5. Hasegawa, M. et al., Nippon Heikatsukin Gakkai
Zasshi, 23(1), 35-46 (1987).
pipette, slowly add dropwise 9 mL Complete ES Media with 15% FBS and LIF
(Catalog No. ES-101-B) (pre-warmed to 37°C) to the 15 mL conical tube. IMPORTANT: Do
not add the whole volume of media at once
pipette, slowly add dropwise 9 mL Complete ES Media with 15% FBS and LIF
(Catalog No. ES-101-B) (pre-warmed to 37°C) to the 15 mL conical tube. IMPORTANT: Do
not add the whole volume of media at once
and mix 10 µL of serum with
1.0 mL of Sample Diluent (101-fold dilution).
Note: Do not dilute the standards, they have
already been pre-diluted 101-fold.
2. Secure the desired number of coated wells
Development 140(5): 1034-1044.
Materials Required But Not Supplied
1. Complete ES Cell Medium with 15% FBS and
LIF (Cat. No. ES-101-B)
2. Recombinant Human bFGF (Cat. No. GF003)
3. Matrigel™
Development 140(5): 1034-1044.
Materials Required But Not Supplied
1. Complete ES Cell Medium with 15% FBS and
LIF (Cat. No. ES-101-B)
2. Recombinant Human bFGF (Cat. No. GF003)
3. Matrigel™
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark
deficient) overnight
at 37°C. Dilute the cells 1/50 in 1% glucose, 1.25 µg/mL thiamine, 0.5 µM
thymidine, 1 mM MgSO4 and 20 µM EdU in M9 minimal media. Grow this culture
at 37°C for 24 hours in the dark