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Showing 31-60 of 714 results for "105-90-8" within Technical Documents
Data Sheet - G0791
For maintaining cultures in Gene Therapy Medium-2,
seed stock cultures at 2 x 105 cells/ml for three days
or at 3 x 105 cells/ml for two days.
NOTE: This maintenance schedule is appropriate for
EX-CELL CD CHO Serum-Free Medium FAQ
Density 3 x 105 cells/mL
Passage frequency 3 - 4 days
Supplements added
at time of use
8 mM L-glutamine
Environment (temperature,
C02, agitation)
37 C (humidified incubator),
8
EX-CELL MDCK Serum-Free Medium for MDCK Cells
serum.
1. Choose cultures in logarithmic growth with viabilities above
90%.
2. Prepare a freezing medium consisting of 90% cold
EX-CELLTM MDCK medium and 10% dimethyl sulfoxide
(DMSO).
3. Using
EX-CELL Sp2 0 Serum-Free Medium for Sp2 0 Cells Chemically Defined
cells from serum-supplemented medium
to EX-CELLTM Sp2/0 supplemented with 8 mM L-glutamine
at a minimum seeding density of 3 x 105 cells/mL in shaker
flasks.
2. Incubate the flasks at 37 C in a humidified
EX-CELL CD CHO Fusion - Chemically defined cell culture medium
by planting new
cultures at 4 x 105 cells/mL in 20–30 mL of
EX-CELL® CD CHO Fusion.
2. Subculture stocks every three to four days at a
seeding density of 4 x 105 cells/mL.
3. Continue stocks
Product Information: EX-CELL®Advanced HD Perfusion Medium
seeded at 3 - 5×105 cells/mL, then subcultured when
densities reach 1 - 2×106 cells/mL and ≥ 80% viability.
Adaptation is completed when cells attain a stable
doubling time and ≥ 90% viability over
EX-CELL CHO Medium (C5467)
viability is at least
90% and the cells are in the mid-logarithmic growt h
phase. Cells grown in serum-containing medium should
be inoculated at a viable cell density of 2 x 105 cells/ml
in a 1:1 mixture
Product Information Sheet - SHM05
0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish 5-10 x
Product Information Sheet - SHM03
0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish 5-10 x
Product Information Sheet - SHM02
0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish 5-10 x
EX-CELL CHO Medium (C1707)
viability is at least
90% and the cells are in the mid-logarithmic growth
phase. Cells grown in serum-containing medium should
be inoculated at a viable cell density of 2 x 105 cells/ml
in a 1:1 mixture
Product Information Sheet - SHM01
0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish 5-10 x
Product Information Sheet - SHM04
0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm dish 5-10 x
EX-CYTE®Growth Enhancement Media Supplement Product Information
10 % FBS in
basal media.
2. Seed culture at 2 × 105 cells/mL or 6 × 104/cm2 and
incubate until the culture reaches 1 × 106 cells/mL or
80 – 90 % confluency.
3. During subculture, reduce
Quality Control Range Sheet- HCY-116 and HCY-216
Eotaxin Control 1 105 – 218 pg/mL IL-13 Control 1 96 – 200 pg/mL
Control 2 494 – 1026 pg/mL Control 2 496 – 1030 pg/mL
Data Sheet - I5408
that cell
viability be >90% and the cells are in mid-logarithmic
growth phase. Cells grown in serum-containing medium
should be inoculated at a viable cell density of >3 x 105
cells/ml in a 1:1 mixture
EX-CELL Sp2 0 Serum-Free Medium for Sp2 0 Cells
cells from serum-supplemented medium
to EX-CELLTM Sp2/0 supplemented with 8 mM L-glutamine
at a minimum seeding density of 3 x 105 cells/mL in shaker
flasks.
2. Incubate the flasks at 37 C in a humidified
Process Guidance Cellvento™ BHK-200 Medium
+ 10% DMSO
– Continue with step 8.
8th adaptation of high density culture in Cellvento™ BHK-200 medium
• Subculture the cells at a seeding density of 6×105 viable cells/mL in Cellvento™ BHK-
Product Information Sheet - 24365C
by planting new cultures
at 4 x 105 cells/mL in 20 - 30 mL of EX-CELLTM CD CHO
Fusion.
2. Subculture stocks every three to four days at a seeding
density of 4 x 105 cells/mL.
3. Continue stocks
Product Information Sheet - 14365C
by planting new cultures at
4 x 105 cells/mL in 20 - 30 mL of EX-CELL CD CHO Fusion.
2. Subculture stocks every three to four days at a seeding
density of 4 x 105 cells/mL.
3. Continue
Product Information Sheet - 14365C
by planting new cultures
at 4 x 105 cells/mL in 20 - 30 mL of EX-CELLTM CD CHO
Fusion.
2. Subculture stocks every three to four days at a seeding
density of 4 x 105 cells/mL.
3. Continue stocks
Process Guidance Cellvento™ CHO-200 Medium
• Prepare the freezing medium by combining Cellvento™ CHO-200 at 90 % with dimethyl sulfoxide (DMSO) at 10 %.
Store at 2–8 °C until use.
• Precipitate cells by centrifugation at 1,000 rpm for
NovAseptic®Mixer, Tank Plate, GMP and HS
0.24)
1.5
(0.06)
4.2
(0.17) GMP5000
GM100/33 85 (3.35)
150
(5.91)
105
(4.13)
81
(3.19)
85
(3.35)
105
(4.13)
20
(0.79)
81
(3.19) M12 45°
125
(4.92) M8
EX-CELL 293 Serum-Free Medium for HEK 293 Cells
disturbing the cell pellet.
3. Resuspend the cells in EX-CELLTM 293 medium at a density
of 6 x 105 cells/mL in shaker flasks.
4. Allow the cells to adapt to EX-CELLTM 293 for an additional
4 - 6
HEK 293 Cell Growth and Virus Production in EX-CELL 293 Serum-Free Medium
cells were seeded at 4 x 105 cells/mL in 125 mL
shaker fl asks containing 35 mL of EX-CELL 293. Flasks
were incubated in a 10% CO2 atmosphere at 37 C, with a
shaker speed of 90 - 100 rpm
EX-CELL VPRO Serum-Free Medium for Retinoblast Cells
1. Subculture the cells from serum-free medium directly into
EX-CELL™ VPRO at a density of 2.5-3 x 105 cells/mL.
2. Allow the cells to adapt to EX-CELL™ VPRO Medium for
an additional 4 - 6 passages.
Data Sheet - A0310
Ophthalmol Vis Sci., 47,
1682-90 (2006).
2. Constantinople CM., et al., J Comp Neurol., 516,
291-311 (2009).
3. Berbari NF., et al., Proc Natl Acad Sci U S A.,
105, 4242-6 (2008).
4. Omelchenko
NovAseptic®Mixer: Tank Plate, GMP ATEX Specification
4.2
(0.17)
GMP5000 ATEX
AX100/33 85
(3.35)
150
(5.91)
105
(4.13)
81
(3.19)
85
(3.35)
105
(4.13)
20
(0.79)
81
(3.19)
M12 45º
Metformin and Related Impurities - SeQuant®ZIC®-cHILIC
&
5 ppm of impurity B in mobile phase
Pressure Drop: 105 bar (1522 psi)
20
10
15
In
te
ns
it
y
(m
V)
0
5
0 4 8 12
Protocol: GyroMark™ HT Rat/ Mouse Cystatin C/Gyros Gyrolab™ xP Workstation Assay
107
4 0.42 0.426 101
8 0.21 0.22 105
16 0.105 0.104 99
32 0.053 0.0603 115
Rat 2 Neat 0.849 0.849
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