Acids, Metals, and Iron 1
B C
0.0E+00
5.0E+05
1.0E+06
1.5E+06
2.0E+06
2.5E+06
3.0E+06
3.5E+06
4.0E+06
4.5E+06
0 1 2 3 4 5 6 7 8 9 1 0 1 1 1 2 1 3
Days
V
ia
b
le
C
el
ls
/m
L
x106 cells/mL with 98.9% viability
5.9E+06
6.9E+06
7.7E+06
7.6E+06
5.5E+06
0.0E+00
5.0E+06
1.0E+07
1.5E+07
Shaker Flasks
SF 1 SF 2 30%
DO, OP
50%
DO, OP
30%
DO, MS
ml
1.0 ml
5 × 1 ml
06 365 779 001
06 365 787 001
06 365 809 001
X-tremeGENE HP DNA Trans-
fection Reagent
0.4 ml
1.0 ml
5 × 1 ml
06 366 244 001
06 366 236
691-695 (1999).
2. Cabantous, S. et al., Protein tagging and detection
with engineered self-assembling fragments of
green fluorescent protein. Nature Biotechnology,
23, 102-107 (2004).
"Fold 'n
691-695 (1999).
2. Cabantous, S. et al., Protein tagging and detection
with engineered self-assembling fragments of
green fluorescent protein. Nature Biotechnology,
23, 102-107 (2004).
"Fold 'n
691-695 (1999).
2. Cabantous, S. et al., Protein tagging and detection
with engineered self-assembling fragments of
green fluorescent protein. Nature Biotechnology,
23, 102-107 (2004).
"Fold 'n
Skyrin
from Penicillium islandicum
Catalog Number SML0459
Storage Temperature -20 °C
CAS RN 602-06-2
Synonyms: Endothianin; Rhodophyscin; Skirin
Product Description
Molecular formula: C30 H18 O10
as much of the
growth medium as possible.
4. Suspend the cells in freezing medium at 106 to
107 cells/ml. Myelomas or hybridomas may require
a higher cell density.
5. Aliquot cells into freezing
as much of the
growth medium as possible.
4. Suspend the cells in freezing medium at 106 to
107 cells/ml. Myelomas or hybridomas may require
a higher cell density.
5. Aliquot cells into freezing
as much of the
growth medium as possible.
4. Suspend the cells in freezing medium at 106 to
107 cells/ml. Myelomas or hybridomas may require
a higher cell density.
5. Aliquot cells into freezing
2005).
4. Vaziri, H., et al., hSIR2-SIRT1 functions as an
NAD-dependent p53 deacetylase. Cell, 107,
149-159 (2001).
5. Vaquero, A., et al., Human SirT1 interacts with
histone H1 and promotes formation
dilution of 1:1,000 is
recommended using human C33A cervical carcinoma
cells. A band of approx imately 107 kDa is detected.
Note: In order to obtain best results in different tech-
niques and preparations
https://www.sigmaaldrich.com
2. How to Use this Product
y Version: 06 Anti-Poly-(ADP-Ribose) Polymerase from rabbit
4 sigma-aldrich.com
2. How to Use this Product
2.1. Before you