heterodimeric structure, consisting of a regulatory 85kD
(p85) subunit and a catalytic 110 kD (p110) subunit.2,5-8
In addition two distinct forms of p85 subunit have been
described: p85� and p85�. The
heterodimeric structure, consisting of a regulatory
85 kDa (p85) subunit and a catalytic 110 kDa (p110)
subunit.2,5-8 In addition two distinct forms of p85 subunit
have been described: p85α and p85β.2 The
sink or aspirating the liquid. Blot the plate(s) on absorbent paper to
remove excess liquid.
8. Add 75 µL per well of the freshly prepared LumiGLO® Chemiluminescent Substrate.
9. Incubate the plate
encodedacetyl transferase of Escherichia coli.
Location of the acetyl group. FEMS Microbiology
Letters 110, pp 331 – 334, 1993.
Notice
We provide information and advice to our customers
on application
to
Cytokines and Their Receptors, Oxford Press (New
York, NY: 1994).
8. Galzie, Z., et al., Biochem. Cell Biol., 75, 669
(1997).
9. Hou, J., et al., J. Biol. Chem., 267, 17804-17808
(1992
Smith, G., and Stoker, C., Arch. Biochem. Biophys.,
21, 383-394 (1949).
16. Holtje, J.V., EXS, 75, 105-110 (1996).
TRITON is a registered trademark of the Union Carbide
Corp.
CS,RBG,ALC,MAM 03/08
broad activity range of pH 4-7.2
Glucose oxidase is specific for β-D-glucose with a Km
of 33-110 mM.5,6
Glucose oxidase does not require any activators.
Inhibitors of glucose oxidase include