was calculated using the Excel file on the ISO
website (http://standards.iso.org/iso/16140)
100% 94%
IMS method
% of recovery Use 2 different selectivity
agar plates
Direct spread of
the enrichment
intracellular protein aggregation
and cell death by an oxygen-dependent
mechanism. FEBS Lett., 542(1-3), 89-94 (2003).
10. Kimura, T., et al., Quantity and quality control of
gastric proton pump in the endoplasmic
sections).
Hypoxia-inducible factor-1 (HIF-1) is a heterodimer
composed of a 120 kDa HIF -1-α subunit complexed
with a 91- to 94 kDa HIF-1-β subunit. The predicted
826-amino acid HIF -1-α contains a bHLH
1,000 extractions
1,000 amplifications
Extraction
Solution E7526 120 ml 120 ml 120 ml
Dilution Solution D5688 120 ml 120 ml 120 ml
Extract-N-Amp
PCR Ready Mix
or SYBR Green
Extract-N-Amp
PCR
PCR conditions were as follows:
42 °C for 15 minutes; 94 °C for 3 minutes (initial denatu-
ration and JumpStart Taq activation); and 45 cycles of 94 °C
15 seconds and 60 °C 1 minute, with data collection
Common cycling parameters
Step Temperature Time Cycles
Initial
Denaturation 94 °C 3 minutes 1
Denaturation 94 °C 30 seconds
30-35
Annealing 45 to 68 °C 30 seconds
Extension 72 °C
Common cycling parameters
Step Temperature Time Cycles
Initial
Denaturation 94 °C 3 minutes 1
Denaturation 94 °C 30 seconds
30-35
Annealing 45 to 68 °C 30 seconds
Extension 72 °C
1-2
wells), prepare
100 µL of Oxidation Reagent Mix by adding 6 µL of
Chloramine T Concentrate to 94 µL of Oxidation Buffer.
Make a sufficient amount of the Oxidation Reaction Mix
for all of the assay
Common cycling parameters
Step Temperature Time Cycles
Initial
Denaturation 94 °C 3 minutes 1
Denaturation 94 °C 30 seconds
30-35
Annealing 45 to 68 °C 30 seconds
Extension 72 °C
Common cycling parameters
Step Temperature Time Cycles
Initial
Denaturation 94 °C 3 minutes 1
Denaturation 94 °C 30 seconds
30-35
Annealing 45 to 68 °C 30 seconds
Extension 72 °C
1-2
the Intestine: A Review. Toxins (Basel), 6(5),
1615-1643 (2014).
3. Sobrova, P. et al., Deoxynivalenol and its toxicity.
Interdiscip. Toxicol., 3(3), 94-99 (2010).
4. Pierron, A. et al., Impact
total of 5 washings.
7. Tap the microwells (face down) on a layer of
absorbent paper to remove residual wash buffer.
8. Measure the required volume of TMB Substrate
(1 mL/strip or
acids which contains an N-terminal catalytic
domain characteristic of STKs.1 STK3 and STK4 share
94% amino acid identity in the catalytic domain and
78% identity overall. RAF1 has been shown to
counteract
acids which contains an N-terminal catalytic
domain characteristic of STKs.1 STK3 and STK4 share
94% amino acid identity in the catalytic domain and
78% identity overall. RAF1 has been shown to
counteract
Common Cycling Parameters
Step Temperature Time Cycles
Initial
Denaturation
94 °C 3 minutes 1
Denaturation 94 °C 15 seconds
– 1 minute
Annealing 45 to 68 °C 15 seconds
– 1 minute
Extension
Common Cycling Parameters
Step Temperature Time Cycles
Initial
Denaturation
94 °C 3 minutes 1
Denaturation 94 °C 15 seconds
– 1 minute
Annealing 45 to 68 °C 15 seconds
– 1 minute
Extension
Figure 5A) or voltage steps to -120 mV (Figure 5B). The half-maximal
inhibition of CEC was found to be between 30 µM and 300 µM consistent with the
published data.
Figure 5. Effect of chloroethylclonidine
cycles.
Figure 1.
SDS-PAGE Gel of Typical Lot
70–95% (densitometry)
PIM1
37
94
130
201
49
Figure 2.
Specific Activity of Typical Lot
269–363 nmole/min/mg
Procedure
Specific Activity of Typical Lot
120 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5 mM
glycerol 2-phosphate, 25 mM MgCl2, 5 mM EGTA, and
2 mM EDTA. Just