slip.
Anti-GSDMD (126-138) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Catalog Number G7422
Product Description
Anti-GSDMD (126-138) is produced in rabbit
the
affinity resin on a plate shaker or an orbital shaker
(approximately 175 rpm) for 15 minutes.
8. Place the plate or tube in the magnetic separator
for 10 seconds. Remove the
Elution Buffer. Mix the
affinity resin on a plate shaker or an orbital
shaker (~175 rpm) for 15 minutes.
8. Place the plate or tube in the magnetic separator
for 10 seconds. Remove the eluate. Save
.
Molecular mass: ∼115 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 102–138 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product is for R&D use only, not for
10614)
Immunogen: Recombinant protein HMBA-inducible
Immunogen Sequence: GI # 5453682, sequence 138 - 359
The product is a clear, colorless solution in phosphate
buffered saline, pH 7.2, containing
that may
be responsible for neurotoxicity.8
Reagent
The antibody is supplied as a solution in 0.01 M
phosphate buffered saline, pH 7.4, containing 15 mM
sodium azide as a preservative.
Antibody
References
1. Steuli, M. et al., EMBO J., 11, 897-907 (1992).
2. Aicher, B. et al., J. Cell. Biol., 138, 681-696 (1997).
3. Kulas, D.T., J. Biol. Chem., 271, 748-754 (1996).
4. Qiao, S. et al., J. Biol
glycosylated. There are two disulfide
bonds for the α-interferons between residues 1 and
98/99 and 29 and 138/139, respectively. Reduction of
sulfhydryls results in the loss of antiviral activity of
leukocyte
USA,
93, 8395 (1996).
7. Hirata, H., et al., J. Exp. Med., 187, 587 (1998).
8. Caulin, C., et al., J. Cell Biol., 138, 1379 (1997).
9. Slee, E. A., et al., J. Cell Biol., 144, 281 (1999)
and to mediate the beneficial
aspects of exercise.8
Reagent
Supplied as a solution in 0.01 M phosphate buffered
saline, pH 7.4, containing 15 mM sodium azide.
Antibody concentration: 1.5
response and mediate the
beneficial aspects of exercise.8
Reagent
Supplied as a solution in 0.01 M phosphate buffered
saline, pH 7.4, containing 15 mM sodium azide.
Antibody concentration: 1.0