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inhibitors. TACE
hydrolyzes a FRET-tagged substrate to release a
fluorescent product (λex = 318/ λem = 449 nm),
proportional to the enzymatic activity present.
Components
The kit is sufficient for 100
d
447 MDM2 M4308 4193 MDM2 M NP_002383.2 y n/d y
448 MDM2 M7815 4193 MDM2 M NP_002383.2 y y n/d
449 MDMX M0445 4194 MDM4 M NP_001191100.1 y n/d n/d
450 MeCP2 M9317 4204 MECP2 P NP_001104262.1,NP_004983.1
d
447 MDM2 M4308 4193 MDM2 M NP_002383.2 y n/d y
448 MDM2 M7815 4193 MDM2 M NP_002383.2 y y n/d
449 MDMX M0445 4194 MDM4 M NP_001191100.1 y n/d n/d
450 MeCP2 M9317 4204 MECP2 P NP_001104262.1,NP_004983.1
d
447 MDM2 M4308 4193 MDM2 M NP_002383.2 y n/d y
448 MDM2 M7815 4193 MDM2 M NP_002383.2 y y n/d
449 MDMX M0445 4194 MDM4 M NP_001191100.1 y n/d n/d
450 MeCP2 M9317 4204 MECP2 P NP_001104262.1,NP_004983.1
MDC1 M2444 M y n/d n/d
446 MDM2 M8558 M n/d y n/d
447 MDM2 M4308 M y y y
448 MDM2 M7815 M y y n/d
449 MDMX M0445 M y n/d n/d
450 MeCP2 M9317 P y n/d n/d
451 MeCP2 M7443 M
006873.3,NP_034916.1 M y y y
448 MDM2 M7815 4193, 17246 MDM2,Mdm2 NP_006873.3,NP_034916.1 M y y n/d
449 MDMX M0445 17248, 304798, 4194 MDM4,Mdm4 NP_032601.2,NP_001012026.1,NP_002384.2 M y n/d n/d
450 MeCP2
006873.3,NP_034916.1 M y y y
448 MDM2 M7815 4193, 17246 MDM2,Mdm2 NP_006873.3,NP_034916.1 M y y n/d
449 MDMX M0445 17248, 304798, 4194 MDM4,Mdm4 NP_032601.2,NP_001012026.1,NP_002384.2 M y n/d n/d
450 MeCP2
.; Ryu, S.; Seo, J.; Seok,
S. I. Nature 2015, 517, 476–480.
(8) Turren-Cruz, S.-H.; Hagfeldt, A.; Saliba, M. Science 2018, 362,
449–453.
(9) Yoo, J. J.; Wieghold, S.; Sponseller, M. C.; Chua, M.
448
3 Establishing the connection and starting the program 449
3.1 Connecting the photometer to the PC . . . . . . . . . . . . . . . 449
3.2 Starting SpectralTransfer . . . .
448
3 Establishing the connection and starting the program 449
3.1 Connecting the photometer to the PC . . . . . . . . . . . . . . . 449
3.2 Starting SpectralTransfer . . . .