cDNA contains a 427
amino acid residue precursor protein with a 28 amino
residue signal peptide, a 222 amino acid residue
extracellular domain, a 22 amino acid residue trans-
membrane domain, and a 155
involving the
obese receptor. J Cell Biochem 114(3):541-50.
Ogawa, T., et al. (2012) MicroRNA-221/222 upregulation
indicates the activation of stellate cells and the progression of
liver fibrosis
purchased from different sources:
Testosterone (NET-553), Propranolol (NET-515) and Digoxin (NET-222) were purchased
from PerkinElmer Life Sciences, Inc. (Boston, MA).
Methotrexate (MT701), Taxol
Pyk2 Phospho (pY
579/580) P6989 Signal Transduction P y n/d n/d
221 Pyk2 Phospho (pY
580) P6739 Signal Transduction P y n/d y
222 Pyk2 Phospho (pY
881) P6864 Signal Transduction P y n/d y
223
..
MEK1 (pSer218/222) from PMA-treated Jurkat cell lysate was serially diluted in
standard dilution buffer. Absorbance of each dilution was plotted against the MEK1
(pSer218/222) standard curve. Demonstrated
Brehm-Gibson T, Murel A. Acid precipitation of
mammalian cell fermentation broth. Ann. NY Acad. Sci. 745,
222–231 (1994).
24 Chen J, Tetrault J, Zhang Y et al. The distinctive separation
attributes of mixed-mode
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�,6-Difluorophthalic anhydride, 97%
C8H2F2O3
O
F
F
O
O
MW: 184.1
MP: 218–221 °C (lit.)
381128-100MG 100 mg 28.20
381128-500MG 500