than 15% cross-reactivity with human
MCP-2 (Cat. No. M-244) and human MCP -3 (Cat. No.
M-245) and less than 5% cross-reactivity with human
MCP-1 (Cat. No. M-243). This antibody shows no
cross-reactivity
86,
8034-8038.
12 Wingfield, P. et al. (1986) Eur. J. Biochem. 160, 491-497.Wingfield,
P. et al. (1986) Eur. J. Biochem. 160, 491-497.
13 Furutani, Y. et al. (1986) Nucleic Acids Res. 14, 3167-
mutorotate to
-D-glucose as the -D-glucose is consumed by the
enzymatic reaction.
1
Molecular mass:
2
160 kDa
(gel filtration)
Glucose oxidase from Aspergillus niger
, (p. 244) 1995.
Improved Performance of M2 Hybridoma Cells
Culture Media in Spinner Flasks
Growth Attribute DMEM H4281 H4281
+10%FBS +2%FBS M2-Optimized
Lag from frozen state 3-4 wks 1-2 days
Chloride Lysis Solution:
160 mM ammonium chloride (Catalog No. A9434), 20 mM sodium bicarbonate
(Catalog No. S5761); pH to 7.2 with HCl.
13
Experienced User Protocol
1 Release DNA from
Chloride Lysis Solution:
160 mM ammonium chloride (Catalog No. A9434), 20 mM sodium bicarbonate
(Catalog No. S5761); pH to 7.2 with HCl.
13
Experienced User Protocol
1 Release DNA from