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Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Non-Fat Dry Milk (NFDM) Solution for Blocking Western Blots

Non-Fat Dry milk (NFDM) buffered solution is ideal for saturating excess protein-binding sites on membranes for Western blotting. Typically, 5% NFDM is sufficient for most Western blots.

Determination of Water Content in Phenol Using Karl Fischer Titration

Accurately measure the moisture content in Phenol (C6H5OH) through Karl Fischer titration, using both Volumetric and Coulometric methods.

Critical Micelle Concentrations (CMCs) - Avanti Research™ Polar Lipids

The critical micelle concentration (CMC) can be approximately defined as the lipid monomer concentration at which appreciable amounts (>5% of total) of micellar aggregates first begin to appear in the equilibrium: nM1Mn

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

Protein Fingerprinting of a Viral Vector, AAV5

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

Purification of NAD+ and ATP-dependent Kinases

Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.

Detection of Signaling Effector-Complexes Downstream of BMP4 Using In Situ PLA, A Proximity Ligation Assay

BMPs are responsible for a wide range of developmental and biological effects. BMP receptors activate (phosphorylate) the Smad1/5/8 effectors, which then, form a complex with Smad4 and translocate to the nucleus where they function as transcription factors to initiate BMP

KAPA3G Plant PCR Kits FAQs

Amplify up to 5 kb fragments from purified plant DNA extracted using commercial kits or CTAB-based methods. Review some of the most asked questions regarding direct PCR from leaf disks, seeds and other plant tissues.

Long and Accurate PCR

Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.

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