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GC Analysis of Sweet Orange Essential Oil on SLB®-5ms (10 m x 0.10 mm I.D., 0.10 µm), Fast GC Analysis
-β-Farnesene; α-Huµlene; Germacrene D; (+)-Valencene; Bicyclogermacrene; (+)-δ-Cadinene
Qualitative Thin Layer Chromatography Analysis Ginkgo
Qualitative Thin Layer Chromatography Analysis of Flavonoids and Quantification of Terpene Lactones in Ginkgo Biloba Extracts and Tablets
Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity
A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.
Analysis of Pesticides in Turmeric Powder by LC/MS/MS and GC/MS/MS after Cleanup with Dual-Layer SPE Cartridge
Pesticide residue testing of turmeric and other (dried) spices is challenging due to their complex compositions and high matrix. An efficient SPE clean-up can be achieved by a new Dual-layer cartridge.
HPLC Separation of Ginsenosides from American Ginseng
The HPLC method for ginsenoside separation was optimized using a ginsenoside standard mixture and then applied to a sample of American Ginseng root. The experimental conditions and chromatograms are shown.
Extractables Studies of Single-Use Equipment Immediate Identification and Quantification of Unknown Extractables by GC/MS with a Certified Reference Material Mix for Extractables and Leachables
Identification and quantification of the most common extractables originating from single-use equipment by GC/MS and an Extractables and Leachables Screening Standard.
Purification of Membrane Proteins
This page shows how to perform a purification of His-tagged membrane proteins.
Resolves Difficult Sample Matrix Problems
In the flavor and fragrance industry, analytical chemists are often required to isolate key ingredients, off-flavor components, or trace organic compounds from their sample matrices without altering their structure or composition.
Thermal Transitions of Homopolymers: Glass Transition & Melting Point
Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.
Photocrosslinkable Hydrogels for 3D Cell Culture and Tissue Engineering
Methacrylated collagen, hyaluronic acid, and gelatin (GelMA) hydrogels can be crosslinked with light and photoinitiators (Irgacure/LAP/Ruthenium), used as 3D cell culture scaffolds and bioinks for bioprinting.
Scorpions® Probes
Scorpions probes are highly sensitive, sequence-specific, bi-labeled fluorescent probe/primer hybrids designed for qPCR.
Quantitative Analysis of Nitrosamine Impurities by LC-MS Methods from the United States Pharmacopeia General Chapter <1469>
This application note describes the LC-MS-based quantitative analysis of known nitrosamine impurities following procedures 1 and 3 as given in USP general chapter .
Performing a Separation with Cytiva Products Based on GST Fusion Proteins
How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from Cytiva.
The Savannah ALD System - An Excellent Tool for Atomic Layer Deposition
igma-Aldrich.com presents an article regarding the savannah ALD system - an excellent tool for atomic layer deposition.
Characteristics of Glutathione Sepharose® Products
Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.
Poly(2-isopropyl-2-oxazoline)-based Multimodal Stimuli-responsive Functional Materials
Based on the temperature properties of PiPrOxs, we have recently developed several multimodal stimuli-responsive polymeric systems. This review will briefly show recent developments of PiPrOx-based functional stimuli-responsive polymers.
Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
How to Make and Use Percoll Gradients
To prepare a Percoll gradient, the osmolality of Percoll must be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
Metal Borohydrides as Hydrogen Storage Materials
An article about metal borohydrides as hydrogen storage materials
radiello® Diffusive Air Sampling Application - Volatile Organic Compounds (VOCs) Thermally Desorbed
Volatile organic compounds are trapped by adsorption and recovered by thermal desorption, analysis is performed by capillary gas chromatography and FID or MS detection.
Peptide Mapping LC-MS/MS Analysis Workflow for Adalimumab
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
Nucleic Acid Sample Preparation Methodology
Spectrophotometry can be used to estimate DNA or RNA concentration and to analyze the purity of the preparation.
Bio-Inspired “Green” Synthesis of Nanomaterials and their Applications
Silica is a very popular inorganic nanomaterial used in a wide range of applications including fillers for rubber, catalyst supports, separation media, carriers in food and agriculture, and abrasive/anticaking agents in cosmetics. It is also widely believed to be an