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Showing 1-30 of 46 results for "40236" within Papers
Vasyl Bondarenko et al.
Biochimica et biophysica acta, 1818(5), 1261-1268 (2012-03-01)
The α4β2 nicotinic acetylcholine receptor (nAChR) is the predominant heteromeric subtype of nAChRs in the brain, which has been implicated in numerous neurological conditions. The structural information specifically for the α4β2 and other neuronal nAChRs is presently limited. In this
Karl B McCann et al.
Biologicals : journal of the International Association of Biological Standardization, 37(1), 32-36 (2008-10-25)
Human serum albumin is a well tolerated therapeutic for the treatment of hypovolemia. Despite all commercial human albumin preparations being derived from plasma, these products can have a highly variable colour. Albumin samples derived from ethanol precipitation and chromatographic fractionation
Daniel Jun et al.
STAR protocols, 3(1), 101044-101044 (2022-01-04)
The formation of defined surfaces consisting of photosynthetic reaction centers (RCs) in biohybrid solar cells is challenging. Here, we start with the production of engineered RCs for oriented binding. RCs are deposited onto gold electrodes, and 6-mercapto-1-hexanol (MCH) is used
Shawn Misialek et al.
Analytical biochemistry, 394(1), 138-140 (2009-07-14)
The hepatitis C virus (HCV) nonstructural protein 3 (NS3) is known to possess multiple enzymatic activities. In addition to its well-characterized protease activity, HCV NS3 also has ATP hydrolase (ATPase) and nucleic acid unwinding (helicase) activities. We systematically studied the
David S White et al.
eLife, 9 (2020-04-09)
Single-molecule approaches provide enormous insight into the dynamics of biomolecules, but adequately sampling distributions of states and events often requires extensive sampling. Although emerging experimental techniques can generate such large datasets, existing analysis tools are not suitable to process the
Shasha Song et al.
The journal of physical chemistry. B, 116(42), 12850-12856 (2012-10-03)
Supramolecular hydrogels were prepared in the mixtures of a chiral amphiphilic lithocholic acid (LCA) and a nonionic surfactant, dodecyldimethylamine oxide (C(12)DMAO), in water. With the addition of LCA to C(12)DMAO micellar solutions, a transition from micelles to gels occurs at
Achim Stocker et al.
Journal of structural biology, 152(2), 140-145 (2005-10-18)
Recently, we reported the cloning of the atp operon encoding for the F(1)F(0)-ATP synthase from the extremely thermoalkaliphilic bacterium Bacillus sp. strain TA2.A1. In this study, the genes encoding the F(1) moiety of the enzyme complex were cloned from the
Xiaoyi Deng et al.
Journal of medicinal chemistry, 57(12), 5381-5394 (2014-05-08)
Malaria is one of the most serious global infectious diseases. The pyrimidine biosynthetic enzyme Plasmodium falciparum dihydroorotate dehydrogenase (PfDHODH) is an important target for antimalarial chemotherapy. We describe a detailed analysis of protein-ligand interactions between DHODH and a triazolopyrimidine-based inhibitor
Aleksandr A Goncharenko et al.
Molecules (Basel, Switzerland), 25(17) (2020-08-23)
The design of cargo carriers with high biocompatibility, unique morphological characteristics, and capability of strong bonding of fluorescent dye is highly important for the development of a platform for smart imaging and diagnostics. In this paper, BODIPY-doped silica nanoparticles were
Chaowei Shi et al.
Protein expression and purification, 68(2), 221-225 (2009-08-01)
In membrane protein biochemical and structural studies, detergents are used to mimic membrane environment and maintain functional, stable conformation of membrane proteins in the absence of lipid bilayers. However, detergent concentration, esp. molar ratio of membrane protein to detergent is
Wenting Wu et al.
Acta crystallographica. Section F, Structural biology communications, 71(Pt 7), 856-860 (2015-07-07)
Rhodopsin is a membrane protein from the G protein-coupled receptor family. Together with its ligand retinal, it forms the visual pigment responsible for night vision. In order to perform ultrafast dynamics studies, a time-resolved serial femtosecond crystallography method is required
Kuo-Hsiang Tang et al.
Biophysical journal, 99(8), 2398-2407 (2010-10-21)
Green photosynthetic bacteria harvest light and perform photosynthesis in low-light environments, and contain specialized antenna complexes to adapt to this condition. We performed small-angle neutron scattering (SANS) studies to obtain structural information about the photosynthetic apparatus, including the peripheral light-harvesting
Kayla D Thompson et al.
Langmuir : the ACS journal of surfaces and colloids, 38(13), 4090-4101 (2022-03-25)
Understanding the interactions between surfactants and proteins is important for the formulation of consumer products as surfactant binding can alter protein activity and stability. Additionally, the structure of the protein-surfactant complex can influence surface activity, which is important for emulsion
Gil Bu Kang et al.
Protein and peptide letters, 14(8), 742-746 (2007-11-06)
Ryanodine receptor 1 (RyR1) is a large homotetrameric calcium channel that plays a pivotal role in skeletal muscle contraction. Sequence comparison and mutagenesis studies indicate that the pore architecture of RyR1, including the last two transmembrane helices and the luminal
Hiroki Konno et al.
The EMBO journal, 25(19), 4596-4604 (2006-09-16)
The chloroplast-type F(1) ATPase is the key enzyme of energy conversion in chloroplasts, and is regulated by the endogenous inhibitor epsilon, tightly bound ADP, the membrane potential and the redox state of the gamma subunit. In order to understand the
Kyung Eun Lee et al.
Colloids and surfaces. B, Biointerfaces, 62(1), 51-57 (2007-11-06)
The integral membrane protein CD40 was found on the surface of B lymphocytes that interact with CD40L on T cells during the immune response. The hydrophobic transmembrane domains of membrane proteins can be stabilized in detergent or in lipid bilayers
Milya Davlieva et al.
Nucleic acids research, 43(9), 4758-4773 (2015-04-22)
LiaR is a 'master regulator' of the cell envelope stress response in enterococci and many other Gram-positive organisms. Mutations to liaR can lead to antibiotic resistance to a variety of antibiotics including the cyclic lipopeptide daptomycin. LiaR is phosphorylated in
Doru Constantin et al.
Physical review letters, 101(9), 098101-098101 (2008-10-15)
We study by synchrotron small-angle x-ray scattering highly aligned lamellar phases of a zwitterionic surfactant, doped with monodisperse and spherical hydrophobic inorganic particles as a function of particle concentration. Analysis of the structure factor of the two-dimensional fluid formed by
Christian D Lorenz et al.
Langmuir : the ACS journal of surfaces and colloids, 27(2), 546-553 (2010-12-21)
A series of large-scale atomistic molecular dynamics simulations were conducted to study the structural and interfacial properties of nonionic dimethyldodecylamine-N-oxide (DDAO) micelles with an aggregation number of 104 in pure water, which was determined using small-angle neutron scattering (SANS). From
Sebastian Hiller et al.
Biomolecular NMR assignments, 4(1), 29-32 (2010-05-04)
The voltage dependent anion channel (VDAC) forms a channel for metabolites and nutrients in the outer membrane of mitochondria, and it is also involved in apoptotic pathways. Here, we report sequence-specific NMR assignments for the isoform 1 of human VDAC
Cheng Ma et al.
PloS one, 10(11), e0143010-e0143010 (2015-11-26)
Membrane proteins play key roles in many biological processes, from acquisition of nutrients to neurotransmission, and are targets for more than 50% of current therapeutic drugs. However, their investigation is hampered by difficulties in their production and purification on a
Debanjan Tewari et al.
Biochimica et biophysica acta, 1848(1 Pt A), 151-158 (2014-12-03)
Voltage dependent anion channel (VDAC) of mitochondria plays a crucial role in apoptosis. Human VDAC-1, reconstituted in planar lipid bilayer showed reduced conductance when treated with curcumin. Curcumin interacts with residues in the α helical N-terminus of VDAC and in
Rie Kakehashi et al.
Journal of oleo science, 58(4), 185-193 (2009-03-14)
The protonation behavior and the stability of micelles of N-lauroylaminopropyl-N',N'- dimethylamine oxide (C12AmCn; n=3) were studied by the hydrogen ion titration and the equilibrium surface tension measurement. The surface potential of C12AmC3 micelles estimated from the results of hydrogen ion
Koji Tanaka et al.
Nature communications, 6, 6337-6337 (2015-02-27)
Pore-forming toxins (PFT) are water-soluble proteins that possess the remarkable ability to self-assemble on the membrane of target cells, where they form pores causing cell damage. Here, we elucidate the mechanism of action of the haemolytic protein fragaceatoxin C (FraC)
Michaela Herrmann et al.
European biophysics journal : EBJ, 44(7), 503-512 (2015-05-29)
Bilayer mechanical properties are not only of crucial importance to the mechanism of action of mechanosensation in lipid membranes but also affect preparative laboratory tasks such as membrane-protein refolding. We report this for coupled refolding and bilayer insertion of outer membrane
Sebastian Hiller et al.
Science (New York, N.Y.), 321(5893), 1206-1210 (2008-08-30)
The voltage-dependent anion channel (VDAC) mediates trafficking of small molecules and ions across the eukaryotic outer mitochondrial membrane. VDAC also interacts with antiapoptotic proteins from the Bcl-2 family, and this interaction inhibits release of apoptogenic proteins from the mitochondrion. We
Stefanie Keis et al.
Journal of bacteriology, 188(11), 3796-3804 (2006-05-19)
The F(1)F(o)-ATP synthases of alkaliphilic bacteria exhibit latent ATPase activity, and for the thermoalkaliphile Bacillus sp. strain TA2.A1, this activity is intrinsic to the F(1) moiety. To study the mechanism of ATPase inhibition, we developed a heterologous expression system in
J Karlovská et al.
Biophysical chemistry, 119(1), 69-77 (2005-10-15)
Sarcoplasmic reticulum Ca-transporting ATPase (EC 3.6.1.38) was isolated from rabbit white muscle, purified and reconstituted into vesicles of synthetic diacylphosphatidylcholines with monounsaturated acyl chains using the cholate dilution method. In fluid bilayers at 37 degrees C, the specific activity of
Thomas Kupke et al.
Communications biology, 3(1), 73-73 (2020-02-16)
Transmembrane signaling proteins play a crucial role in the transduction of information across cell membranes. One function of regulated intramembrane proteolysis (RIP) is the release of signaling factors from transmembrane proteins. To study the role of transmembrane domains (TMDs) in modulating
Masateru Okazaki et al.
Biochimica et biophysica acta, 1848(12), 3188-3196 (2015-09-27)
The voltage-dependent anion channels (VDACs), VDAC1, VDAC2, and VDAC3, are pore-forming proteins that control metabolite flux between mitochondria and cytoplasm. VDAC1 and VDAC2 have voltage-dependent gating activity, whereas VDAC3 is thought to have weak activity. The aim of this study
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