45533

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Showing 1-30 of 122 results for "45533" within Papers

Multiresidue analysis of pollutants as their trimethylsilyl derivatives, by gas chromatography-mass spectrometry

Sebok A, et al.

Journal of Chromatography A, 1216(12), 2288-2301 (2009)

Rapid and generic identification of influenza A and other respiratory viruses with mass spectrometry.

Joanna A Majchrzykiewicz-Koehorst et al.

Journal of virological methods, 213, 75-83 (2014-12-17)

The rapid identification of existing and emerging respiratory viruses is crucial in combating outbreaks and epidemics. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid and reliable identification method in bacterial diagnostics, but has not been used in

Copper mediates auxin signalling to control cell differentiation in the copper moss Scopelophila cataractae.

Toshihisa Nomura et al.

Journal of experimental botany, 66(5), 1205-1213 (2014-11-28)

The copper (Cu) moss Scopelophila cataractae (Mitt.) Broth. is often found in Cu-enriched environments, but it cannot flourish under normal conditions in nature. Excess Cu is toxic to almost all plants, and therefore how this moss species thrives in regions

A role for a dioxygenase in auxin metabolism and reproductive development in rice.

Zhigang Zhao et al.

Developmental cell, 27(1), 113-122 (2013-10-08)

Indole-3-acetic acid (IAA), the natural auxin in plants, regulates many aspects of plant growth and development. Extensive analyses have elucidated the components of auxin biosynthesis, transport, and signaling, but the physiological roles and molecular mechanisms of auxin degradation remain elusive.

Surgery for simple and complex subaortic stenosis in children and young adults: results from a prospective, procedure-based national database.

Dan M Dorobantu et al.

The Journal of thoracic and cardiovascular surgery, 148(6), 2618-2626 (2014-08-27)

To identify the outcomes of surgically treated subaortic stenosis in a national population. From 2000 to 2013, 1047 patients aged < 40 years underwent 1142 subaortic stenosis procedures. Of the 1047 patients, 484 (46.2%) were considered to have complex stenosis

Metrological traceability in mass spectrometry-based targeted protein quantitation: a proof-of-principle study for serum apolipoproteins A-I and B100.

Nico P M Smit et al.

Journal of proteomics, 109, 143-161 (2014-06-28)

In this study, we have followed up on previous liquid chromatography (LC) multiple reaction monitoring (MRM) mass spectrometry (MS) approaches for measurement of apolipoprotein (apo) A-I and apo B100 in serum aiming for implementation of a multiplexed assay in a

The extended version of restriction analysis approach for the examination of the ability of low-molecular-weight compounds to modify DNA in a cell-free system.

Dominik Kołodziejski et al.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 75, 118-127 (2014-12-03)

One of the primary requirements in toxicology is the assessment of ability of chemicals to induce DNA covalent modification. There are several well-established methods used for this purpose such as (32)P-Postlabeling or HPLC-MS. However, all of these approaches have difficult

Investigation of bi-enzymatic reactor based on hybrid monolith with nanoparticles embedded and its proteolytic characteristics.

Lulu Shangguan et al.

Journal of chromatography. A, 1388, 158-166 (2015-03-03)

The bottom-up strategy of proteomic profiling study based on mass spectrometer (MS) has drawn high attention. However, conventional solution-based digestion could not satisfy the demands of highly efficient and complete high throughput proteolysis of complex samples. We proposed a novel

In-depth analysis of site-specific N-glycosylation in vitronectin from human plasma by tandem mass spectrometry with immunoprecipitation.

Heeyoun Hwang et al.

Analytical and bioanalytical chemistry, 406(30), 7999-8011 (2014-11-07)

The characterization of site-specific microheterogeneity in glycoprotein is very important for understanding cell biology and disease processes. Vitronectin is well known to be a multifunctional glycoprotein in the blood and the extracellular matrix, which is related to hepatocellular carcinoma (HCC).

Interactions between indole-3-acetic acid (IAA) with a lectin from Canavalia maritima seeds reveal a new function for lectins in plant physiology.

Plinio Delatorre et al.

Biochimie, 95(9), 1697-1703 (2013-06-04)

Indole-3-acetic acid (IAA) bound is considered a storage molecule and is inactive. However, some studies have proposed an additional possible regulatory mechanism based on the ability of lectins to form complexes with IAA. We report the first crystal structure of

Regulation of wheat seed dormancy by after-ripening is mediated by specific transcriptional switches that induce changes in seed hormone metabolism and signaling.

Aihua Liu et al.

PloS one, 8(2), e56570-e56570 (2013-02-26)

Treatments that promote dormancy release are often correlated with changes in seed hormone content and/or sensitivity. To understand the molecular mechanisms underlying the role of after-ripening (seed dry storage) in triggering hormone related changes and dormancy decay in wheat (Triticum

Accelerated recovery of mitochondrial membrane potential by GSK-3β inactivation affords cardiomyocytes protection from oxidant-induced necrosis.

Daisuke Sunaga et al.

PloS one, 9(11), e112529-e112529 (2014-11-13)

Loss of mitochondrial membrane potential (ΔΨm) is known to be closely linked to cell death by various insults. However, whether acceleration of the ΔΨm recovery process prevents cell necrosis remains unclear. Here we examined the hypothesis that facilitated recovery of

Analysis of peptides and protein digests by reversed phase high performance liquid chromatography-electrospray ionisation mass spectrometry using neutral pH elution conditions.

Yuanzhong Yang et al.

Analytica chimica acta, 872, 84-94 (2015-04-22)

In this study, the advantages of carrying out the analysis of peptides and tryptic digests of proteins under gradient elution conditions at pH 6.5 by reversed-phase liquid chromatography (RP-HPLC) and in-line electrospray ionisation mass spectrometry (ESI-MS) are documented. For these

Binding of ring-substituted indole-3-acetic acids to human serum albumin.

Milan Soskić et al.

Bioorganic & medicinal chemistry, 15(13), 4595-4600 (2007-05-08)

The plant hormone, indole-3-acetic acid (IAA), and its ring-substituted derivatives have recently attracted attention as promising pro-drugs in cancer therapy. Here we present relative binding constants to human serum albumin for IAA and 34 of its derivatives, as obtained using

The transcriptional regulator BBX24 impairs DELLA activity to promote shade avoidance in Arabidopsis thaliana.

Carlos D Crocco et al.

Nature communications, 6, 6202-6202 (2015-02-07)

In response to canopy shade, plant vegetative structures elongate to gain access to light. However, the mechanism that allows a plastic transcriptional response to canopy shade light is not fully elucidated. Here we propose that the activity of PIF4, a

Uremic serum and solutes increase post-vascular interventional thrombotic risk through altered stability of smooth muscle cell tissue factor.

Vipul C Chitalia et al.

Circulation, 127(3), 365-376 (2012-12-28)

Stent thrombosis (ST), a postinterventional complication with a mortality rate of 50%, has an incidence that rises precipitously in patients at risk. Chronic renal failure and end-stage renal disease have emerged as particularly strong ST risk factors, yet the mechanism

Humic Matter in Soil and the Environment: Principles and Controversies (2003)

Simple analytical strategy for MALDI-TOF-MS and nanoUPLC-MS/MS: quantitating curcumin in food condiments and dietary supplements and screening of acrylamide-induced ROS protein indicators reduced by curcumin.

Yu-Shu Huang et al.

Food chemistry, 174, 571-576 (2014-12-23)

Curcumin is the major active ingredient of turmeric and is widely used as a preservative, flavouring and colouring agent. Curcumin is a potent substance with several functions, including antioxidant, antitumor, anti-inflammatory, antimicrobial, antiparasitic, antimutagenic, chemopreventive and chemotherapeutic activities. Matrix-assisted laser

Plant growth-promoting potential of bacteria isolated from active volcano sites of Barren Island, India.

N Amaresan et al.

Letters in applied microbiology, 58(2), 130-137 (2013-10-19)

To elucidate the biodiversity of plant growth-promoting (PGP) bacteria in active volcano sites of Barren Island, India, a total of 102 bacteria were isolated and screened for their multifunctional PGP properties. The results revealed that 21 isolates (20.6%) survived heat

An integrated quantitative and targeted proteomics reveals fitness mechanisms of Aeromonas hydrophila under oxytetracycline stress.

Xiangmin Lin et al.

Journal of proteome research, 14(3), 1515-1525 (2015-01-27)

To date, above ten thousand tons of antibiotics are used in aquaculture each year that lead to the deterioration of natural resources. However, knowledge is limited on the molecular biological behavior of common aquatic pathogens against antibiotics stress. In this

Quantitative analysis of N-glycans from human alfa-acid-glycoprotein using stable isotope labeling and zwitterionic hydrophilic interaction capillary liquid chromatography electrospray mass spectrometry as tool for pancreatic disease diagnosis.

Estela Giménez et al.

Analytica chimica acta, 866, 59-68 (2015-03-04)

In this work we demonstrate the potential of glycan reductive isotope labeling (GRIL) using [(12)C]- and [(13)C]-coded aniline and zwitterionic hydrophilic interaction capillary liquid chromatography electrospray mass spectrometry (μZIC-HILIC-ESI-MS) for relative quantitation of glycosylation variants in selected glycoproteins present in

Stress resistance of Escherichia coli and Bacillus subtilis is modulated by auxins.

J Repar et al.

Canadian journal of microbiology, 59(11), 766-770 (2013-11-12)

Two bacterial species, Gram-negative Escherichia coli and Gram-positive Bacillus subtilis, were exposed to different auxins to examine possible effects of these substances on bacterial stress tolerance. Bacterial resistance to UV irradiation, heat shock, and streptomycin was assessed with and without

Quantifying protein measurands by peptide measurements: where do errors arise?

Irene van den Broek et al.

Journal of proteome research, 14(2), 928-942 (2014-12-17)

Clinically actionable quantification of protein biomarkers by mass spectrometry (MS) requires analytical performance in concordance with quality specifications for diagnostic tests. Laboratory-developed tests should, therefore, be validated in accordance with EN ISO 15189:2012 guidelines for medical laboratories to demonstrate competence

Substrate promiscuity of the cyclic dipeptide prenyltransferases from Aspergillus fumigatus ( section sign).

Huixi Zou et al.

Journal of natural products, 72(1), 44-52 (2008-12-31)

This study reports that a series of tryptophan derivatives with modifications on the side chain or at the indole ring were accepted by two cyclic dipeptide prenyltransferases, CdpNPT and FtmPT1, and converted to prenylated derivatives. The structures of the enzymatic

Hepatitis B virus e antigen (HBeAg) may have a negative effect on dendritic cell generation.

Ibrahim Hatipoglu et al.

Immunobiology, 219(12), 944-949 (2014-08-26)

Hepatitis B virus (HBV) continues to be a serious worldwide health problem despite the use of protective HBV vaccines and therapeutic regimens against chronic HBV infection. Chronic HBV patients cannot induce sufficient immune responses against the virus. HBV and its

The gut microbiota modulates glycaemic control and serum metabolite profiles in non-obese diabetic mice.

Thomas U Greiner et al.

PloS one, 9(11), e110359-e110359 (2014-11-13)

Islet autoimmunity in children who later progress to type 1 diabetes is preceded by dysregulated serum metabolite profiles, but the origin of these metabolic changes is unknown. The gut microbiota affects host metabolism and changes in its composition contribute to

Consequences of a deficit in vitamin B6 biosynthesis de novo for hormone homeostasis and root development in Arabidopsis.

Svetlana Boycheva et al.

Plant physiology, 167(1), 102-117 (2014-12-06)

Vitamin B(6) (pyridoxal 5'-phosphate) is an essential cofactor of many metabolic enzymes. Plants biosynthesize the vitamin de novo employing two enzymes, pyridoxine synthase1 (PDX1) and PDX2. In Arabidopsis (Arabidopsis thaliana), there are two catalytically active paralogs of PDX1 (PDX1.1 and

Non-rhizobial endophytic (NRE) yeasts assist nodulation of Rhizobium in root nodules of blackgram (Vigna mungo L.).

Kalyanasundaram Geetha Thanuja et al.

Archives of microbiology, 202(10), 2739-2749 (2020-08-02)

The signal orchestration between legumes and the rhizobia attribute to symbiotic nitrogen fixation through nodule formation. Root nodules serve as a nutrient-rich reservoir and harbor diverse microbial communities. However, the existence of non-rhizobial endophytes (NRE) and their role inside the

Distinct protein signature of hypertension-induced damage in the renal proteome of the two-kidney, one-clip rat model.

Heidrun Vethe et al.

Journal of hypertension, 33(1), 126-135 (2014-10-12)

Hypertensive nephrosclerosis is one of the most frequent causes of chronic kidney failure. Proteome analysis potentially improves the pathophysiological understanding and diagnostic precision of this disorder. In the present exploratory study, we investigated experimental nephrosclerosis in the two-kidney, one-clip (2K1C)

The Asymmetric Expression of SAUR Genes Mediated by ARF7/19 Promotes the Gravitropism and Phototropism of Plant Hypocotyls.

Xiaoyi Wang et al.

Cell reports, 31(3), 107529-107529 (2020-04-23)

The asymmetric distribution of auxin leads to the bending growth of hypocotyls during gravitropic and phototropic responses, but the signaling events downstream of auxin remain unclear. Here, we identify many SAUR genes showing asymmetric expression in soybean hypocotyls during gravistimulation

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