by mixing 600 µL milk with 100 µL of
6 N HCl.
2. Centrifuge mixture for 5 minutes at 14,000 rpm.
3. Transfer 300 µL supernatant into a clean tube and
neutralize
temperature, with shaking (600 rpm).
5. Remove liquid from all wells. Wash wells three
times with 300 µL of 1x wash buffer (see Reagent
Preparation). Blot on absorbent paper towels.
6. Add 100 µL of anti-alpha
hydrolysis of conjugated steroid
metabolites to be a one-hour incubation at 55 °C at
pH 5.2, using 600 units of enzyme per mL of urine.4
β-Glucuronidase Type L-II from keyhole limpet is a
crude
Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
XNAS2pis Rev 08/22 6
Notice
We provide information and advice to our customers
on
Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
XNAS2pis Rev 08/22 6
Notice
We provide information and advice to our customers
on
Digest
Enzyme
CS0006C 1 mL Red cap/ vial
30× Probe CS0006D 600 µL Brown vial
10×
Development
Solution
CS0006E 600 µL Brown vial
Component Information
Assay Buffer (Component
activity.
See Asay Procedure, Step 6.
Mix 60 µL of the substrate and 60 µL of the Chromogen for
each well used. Example: for 10 wells, mix 600 µL of
substrate and 600 µL of Chromogen.
the induced and the control cells by
centrifugation at 600 X g for 5 min at 4°C.
5. Remove the supernatant entirely by gentle suction.
6. Suspend the cells in 1X binding buffer at a
into the well according to
the following volumes:
• 800 μL for soybean or similar sized seeds
• 600 μL for cotton or similar sized seeds
• 200 μL for canola, sorghum, wheat, or similar
sized
into the well according to
the following volumes:
• 800 μL for soybean or similar sized seeds
• 600 μL for cotton or similar sized seeds
• 200 μL for canola, sorghum, wheat, or similar
sized
anti-cytokine
autoantibody levels. Anti-IL-6 and anti-IL-22 antibodies have also been associated with severe and recurrent
bacterial infections.
0 200 400 600 800 1000 1200 1400 1600 1800 2000
Sample
Centrifuge the induced and the control cells by
centrifugation at 600 X g for 5 min at 4°C.
5. Remove the supernatant entirely by gentle suction.
6. Suspend the cells in 1X binding buffer at a
concentration
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NOTE: This user guide is also available in the following
Petri Dish without pad 150 PD2004700
Petri Dish without pad 600 PD2004705
Petri Dish with pad 150 PD20047S0
Petri Dish with pad 600 PD20047S5
10 canisters of 100 absorbent pads 1000 AP10045S0
of water. Dilute the pellet until the OD800
is 600 µL of 5 mg/mL MycoPolyzyme to 13 mL
of diluted culture.*
6. Incubate the sample at 35 °C, 250 rpm, along
with a negative control
Petri Dish without pad 150 PD2004700
Petri Dish without pad 600 PD2004705
Petri Dish with pad 150 PD20047S0
Petri Dish with pad 600 PD20047S5
10 canisters of 100 absorbent pads 1000 AP10045S0
Petri Dish without pad 150 PD2004700
Petri Dish without pad 600 PD2004705
Petri Dish with pad 150 PD20047S0
Petri Dish with pad 600 PD20047S5
10 canisters of 100 absorbent pads 1000 AP10045S0