chapter 621).
Internal standard solution: Prepare a solution of benzoic acid in methanol containing about 6 mg per mL.
Solvent mixture: Prepare a mixture of methanol and glacial acetic acid (95:5).
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16 h
at 4°C in 66 mM Tris-HCl, 5 mM MgCl2, 5 mM Dithioth-
reitol, 1 mM ATP, pH 7.5 (at 20°C) resulting in >95 %
recovery of 1 μg �DNA fragments.
Subsequent re-cutting with Pst I yields > 95%
mixture of 0.025 M Ammonium acetate solution and methanol (95:5).
Make adjustments if necessary (see System Suitability under Chromatography 621).
System suitability solutionSystem suitability solution
mixture of 0.025 M Ammonium acetate solution and methanol (95:5).
Make adjustments if necessary (see System Suitability under Chromatography 621).
System suitability solutionSystem suitability solution
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chapter 621).
Internal standard solution: Prepare a solution of benzoic acid in methanol containing about 6 mg per mL.
Solvent mixture: Prepare a mixture of methanol and glacial acetic acid (95:5).
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