Phillips, N. E., and Parker, D. C., J. Immunol., 132,
627-632 (1984).
6. Choquet, D., et al., J. Cell Biol., 121, 355-363
(1993).
7. Tridandapani, S., et al., J. Immunol., 158, 1125-
1132 (
medium.
7. Plate cells to T75 flask, culture cells at 5% CO2, 37°C tissue
culture incubator.
8. Change medium every day.
9. Cells are ready to be passage upon 70-90%
.0 area ratio (lower left
graphs, Figure 7 and Figure 8) to the
freshness of Feed 3 (hours old rather than
days). Feed 2 at pH 6.5 achieved 400-L/m2
with 90% flow decay, whereas Feed 3 at
pH 6.5
et al. Biosens. Bioelectron. �00�, 17, 53. (6) Brahim, S. et al.
Electroanalysis. �00�, 14(9), 627. (7) Rubio Retama, J. et al. Biosens. Bioelectron.
�004, 20, 1111. (8) Brahim, S. et al. Microchimica
regiorandom)
��90�7 (electronic grade)
-3.2 eV -5.1 eV
PCBM
n-type
�591�9
-3.7 eV -6.1 eV
Prod. No. Fullerene Type Structure
��818� N-methylfulleropyrrolidine
(MP-C60)
N CH3
�588�7 (1,2
spectrometry” Bernd O. Keller, Jie Sui, Alex B. Young and Randy M. Whittal
Analytica Chimica Acta 627, Issue 1, 3 October 2008, Pages 71-81. However, we have updated
the masses listed in the previous
representative MAPs is
related to loading volume of plasma samples.
>90% of all 10 MAPs can be depleted from 50 µL
human plasma.
Figure 7. run-to-run depletion consistency
of supermix columns
Three
5747 PTK2 P NP_001186578.1 y y y
266 phospho-FAK (pTyr397) F7926 5747 PTK2 P NP_001186578.1 y y y
627 RAD1 R5029 5810 RAD1 P NP_002844.1 y n/d n/d
556 Paxillin P1093 5829 PXN M NP_001074324.1 y n/d y
623
5747 PTK2 P NP_001186578.1 y y y
266 phospho-FAK (pTyr397) F7926 5747 PTK2 P NP_001186578.1 y y y
627 RAD1 R5029 5810 RAD1 P NP_002844.1 y n/d n/d
556 Paxillin P1093 5829 PXN M NP_001074324.1 y n/d y
623
5747 PTK2 P NP_001186578.1 y y y
266 phospho-FAK (pTyr397) F7926 5747 PTK2 P NP_001186578.1 y y y
627 RAD1 R5029 5810 RAD1 P NP_002844.1 y n/d n/d
556 Paxillin P1093 5829 PXN M NP_001074324.1 y n/d y
623
spectrometry” Bernd O. Keller, Jie Sui, Alex B. Young and Randy M. Whittal
Analytica Chimica Acta 627, Issue 1, 3 October 2008, Pages 71-81. However, we have updated
the masses listed in the previous