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Faster Protein and Peptide Liquid Chromatography (FP2LC)

Larger porous shell particles with narrow particle size distribution, used in Supelco's BIOshell™ columns for the reversed-phase U/HPLC analysis of peptides and proteins, provide increased efficiency per unit pressure drop.

Derivatization and Separation of Aliphatic Amines

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

Absorbance to Transmittance Converter

Convert absorbance to transmittance values and vice versa.

Purification or Removal of Proteins and Peptides

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

Performing a Purification of NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ Using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B

This page shows how to purify NADP+-dependent dehydrogenases and other enzymes with affinity for NADP+ by affinity chromatography using 2’5’ ADP Sepharose 4B and Red Sepharose CL-6B from Cytiva.

Hexafluoroalcohol-functionalized Methacrylate Monomers for Lithographic/nanopatterning materials

To keep pace with Moore′s Law, there is a continuing need in the semiconductor industry to achieve higher circuit density in microelectronic devices.

Immunohistochemistry: Current Applications in Breast Cancer

Immunohistochemistry (IHC) is used to characterize intracellular proteins or various cell surfaces in all tissues. Individual markers or more often panels of various marker proteins can be used to characterize various tumor subtypes, confirm tissue of origin, distinguish metastatic from

Mouse Embryonic Stem Cell Culture Procedures & Protocols

Stem Cell protocols for cryopreservation, thawing of cryopreserved stem cells and media preparation.

Materials for Clean H² Production from Bioethanol Reforming

Hydrogen is one of the most important resources in providing food, fuel, and chemical products for our everyday life. Sustainable catalytic hydrogen production from bioethanol has gained significant attention in recent years due to globally diminishing fossil fuel supplies, which

Preactivated Magnetic Beads for Affinity Chromatography

Covalent coupling of antibodies, aptamers, and proteins with a NHS Mag Sepharose, Sera-Mag Carboxylate-Modiﬁed, Sera-Mag SpeedBeads Carboxylate-Modiﬁed from Cytiva.

Metal Borohydrides as Hydrogen Storage Materials

An article about metal borohydrides as hydrogen storage materials

Bio-Inspired “Green” Synthesis of Nanomaterials and their Applications

Silica is a very popular inorganic nanomaterial used in a wide range of applications including fillers for rubber, catalyst supports, separation media, carriers in food and agriculture, and abrasive/anticaking agents in cosmetics. It is also widely believed to be an

Properties of Mononuclear Cells Isolated by the Ficoll-Paque Method

The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.

UPS1 & UPS2 Proteomic Standards

Standardize Your Research. We offer both the Universal Proteomics Standard and the Proteomics Dynamic range Standard as complex, well-defined, well characterized reference standards for mass spectrometry.

Chromatofocusing: Principles and Methods

This page covers the principles and methods of chromatofocusing, a chromatography technique that separates proteins according to differences in their isoelectric point (pI).

Solubility Table of Compounds in Water at Temperature

Check the solubility of 128 common inorganic compounds (salts and acids) in water at different temperatures from 0 degrees Celsius to 100 C. Compound formulas are also given in this table of solubility in relation to temperature.

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Frequently Asked Questions About Chromatin Immunoprecipitation (ChIP)

See all frequently asked questions (FAQ’s) about chromatin immunoprecipitation (ChIP).

Self-Propagating Reactions Induced by Mechanical Alloying

An article concerning self-propagating reactions induced by mechanical alloying, presented by Sigma-Aldrich.com.

The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.

Organic Semiconductor Laser Materials

HPLC Method Transfer Calculator

Easily transfer a method between HPLC columns, (scale between microbore through preparative range), based on the 2 column dimensions and current method conditions.

Immunohistochemistry Applications in Gastrointestinal Cancer

Colorectal cancer is the third most common cancer in both men and women. An estimated 136,000 cases of colorectal cancer are expected to occur in 2016.

3D Cell Culture with Corning Microplates

Take a deep dive into the world of 3D cell culture courtesy of Corning and understand why the Corning® spheroid microplate can be an asset in the evolution of 3D cell culture.

Functionalized PEI and Its Role in Gene Therapy

Gene therapy has become one of the most discussed techniques in biomedical research in recent years.

2D MXenes Ink: Synthesis, Properties, and Their Precursors

Optimizing the synthesis of high-quality 2D MXene flakes for 3D ink printing is essential to such technological developments as printable and flexible electronics.

Nanocomposite Coatings with Tunable Properties Prepared by Atomic Layer Deposition

Immunohistochemistry: Current Applications in Skin Cancer

Immunohistochemistry (IHC) techniques and applications have greatly improved, dermatopathology is still largely based on H&E stained slides.This paper outlines ways in which IHC antibodies can be utilized for dermatopathology.

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