is necessary for the execution of the muscle
program. Molec. Cell, 4, 725-734 (1999).
RC,MAM 11/11-1
PCAF
34
43
72
95
130
170
56
26
2011 Sigma-Aldrich Co. LLC. All rights reserved
315 959 001
100 ml, 4 x 25 ml 03 315 843 001
SuRE/Cut Buffers SuRE/Cut Buffer A, 5 x 1 ml 11 417 959 001
SuRE/Cut Buffer M, 5 x 1 ml 11 417 983 001
SuRE/Cut Buffer H, 5 x
volume each
11 585 550 910
Taq DNA Polymerase, 1 U/µl 250 U, 1 U/μl
200 reactions in a final volume of 50 μl
11 647 679 001
1,000 U, 4 x 250 U
800 reactions in a
2 min denaturation
at 95°C.
B: without treatment
Lanes 1, 7, 13: DNA molecular weight marker VI
Lanes 2, 8: 1 ng DNA
Lanes 3, 9: 10 pg DNA
Lanes 4, 10: 0.1 pg DNA
Lanes 5, 11: 1 fg DNA
Lanes 6, 12: control
60 minutes ZFF 23 °C Yes
3 30 minutes 70% Alcohol 23 °C No
4 30 minutes 80% Alcohol 23 °C No
5 30 minutes 95% Alcohol 23 °C No
6 30 minutes 95% Alcohol 23 °C No
7 60 minutes 100% Alcohol 23 °C
Recent advances in amyotrophic
lateral sclerosis. Current Opinion in Neurology,
13(4), 397–405 (2000).
RC,MAM 11/11-1
2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark
HePTP. Oncogene, 19, 858-869
(2000).
BIOMOL Green is a trademark of Enzo Life Sciences.
FF,MAM 11/11-1
2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼150 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 95–129 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
This product
kinase.
Nature, 411, 102-107 (2001).
BIOMOL Green is a trademark of Enzo Life Sciences.
FF,MAM 11/11-1
2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
/-
BALB/c mice suggests a pathogenic role of Th1
cells. J. Immun., 172, 97-103 (2004).
RC,MAM 11/11-1
2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
kinase.
Nature, 411, 102-107 (2001).
BIOMOL Green is a trademark of Enzo Life Sciences.
FF,MAM 11/11-1
2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich
Proc. Natl. Acad. Sci. USA, 95, 3792
(1998).
11. Lee, F.S., et al., Proc. Natl. Acad. Sci. USA, 95,
9319 (1998).
12. Nakano, H., et al., Proc. Natl. Acad. Sci. USA, 95,
3537 (1998).
13. Takeda
Proc. Natl. Acad. Sci. USA, 95, 3792
(1998).
11. Lee, F.S., et al., Proc. Natl. Acad. Sci. USA, 95,
9319 (1998).
12. Nakano, H., et al., Proc. Natl. Acad. Sci. USA, 95,