and localized
at human telomeres.11
Reagent
Bovine PARP-1, 98% is supplied as 10 µg protein in
50 mM Tris-HCl, pH 7.4, 1 M NaCl, 10 mM β-mercapto-
ethanol, with 10 mM 3-methoxybenzamidine in DMSO.
pH-dependent. The pKa of
iminobiotin is in the range of 11-12, with a KD = 3.5 x 10
-11 in base, but at pH 3-4, the KD of the protonated iminobiotin
3 .2
>C=NH
then equilibrate the column with 2 column volumes of starting
buffer.
P7700 Page 3 of 5
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PROTEIN G IMMOBILIZED ON AGAROSE
USAGE: (continued)
Do
then equilibrate the column with 2 column volumes of starting
buffer.
P7700 Page 3 of 5
11/23/98 - LMY
PROTEIN G IMMOBILIZED ON AGAROSE
USAGE: (continued)
Do
then equilibrate the column with 2 column volumes of starting
buffer.
P7700 Page 3 of 5
11/23/98 - LMY
PROTEIN G IMMOBILIZED ON AGAROSE
USAGE: (continued)
Do
then equilibrate the column with 2 column volumes of starting
buffer.
P7700 Page 3 of 5
11/23/98 - LMY
PROTEIN G IMMOBILIZED ON AGAROSE
USAGE: (continued)
Do
then equilibrate the column with 2 column volumes of starting
buffer.
P7700 Page 3 of 5
11/23/98 - LMY
PROTEIN G IMMOBILIZED ON AGAROSE
USAGE: (continued)
Do
(2001).
10. Nelson JW., et al., FEMS Immun Med Microbiol.,
8: 89‐98 (1994).
11. Kwak Y. and Shin JH., J Biotechnol., 10:
3-4 (2015).
Notice
We provide information and advice to our customers
STE (Cat. Nos. 11 273 922 001, 11 273 949 001) or TE (Cat.
No. 11 522 990 001§) buffer; G-50 columns have an exclusion limit of 72
bases and are available in STE (Cat. Nos. 11 273 965 001, 11 273