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Showing 1-30 of 33 results for "B4136" within Papers
René-Peiman Zahedi et al.
Proteomics, 5(14), 3581-3588 (2005-08-03)
Despite the importance of membranes in any living system, the global analysis of membrane subproteomes is still a common obstacle. In particular, the widely used 2-DE technique consisting of IEF in the first dimension and SDS-PAGE in the second dimension
Tom M H Choy et al.
Electrophoresis, 24(17), 3040-3048 (2003-09-16)
New insights into the effects of the wall adsorption of the visualization agent on baseline noise characteristics in indirect UV detection in capillary electrophoresis were provided. When compared to relatively small, hydrophilic cationic (1-(4-pyridyl)pyridinium chloride hydrochloride) or anionic chromophores (bromide
R Henkel et al.
Biological chemistry Hoppe-Seyler, 373(8), 685-689 (1992-08-01)
Flagella of human spermatozoa were separated from the sperm head by sonication at 25 kHz and subsequent density gradient centrifugation in Percoll. For isolation of the outer dense fibers (ODF), the flagellar membrane and fibrous sheath were dissolved under reducing
J Sánchez-Martín et al.
Journal of hazardous materials, 174(1-3), 9-16 (2009-09-29)
Natural tannin-based adsorbent has been prepared on the basis of the gelification of Quebracho bark extract. The resulting product, Quebracho Tannin Gel (QTG) was tested as cationic dye adsorbent with Methylene Blue (MB). Kinetics of adsorption process were studied out
Joe J Harrison et al.
Antimicrobial agents and chemotherapy, 52(8), 2870-2881 (2008-06-04)
Biofilms are slimy aggregates of microbes that are likely responsible for many chronic infections as well as for contamination of clinical and industrial environments. Pseudomonas aeruginosa is a prevalent hospital pathogen that is well known for its ability to form
Francisco Javier Arrebola-Liébanas et al.
Journal of AOAC International, 97(4), 1021-1026 (2014-08-26)
A simple and fast method has been developed for determining relevant quaternary ammonium compounds in cucumber and orange samples. The target compounds were benzoalkonium chloride (BAC-10, BAC-12, BAC-14, and BAC-16), didecyldimethylammonium chloride, and benzethonium chloride, all frequently used biocides in
H Liang et al.
Molecular vision, 14, 204-216 (2008-03-19)
To evaluate and compare the toxicological profiles of two quaternary ammonium compounds (QAC), benzalkonium chloride (BAK), and cetalkonium chloride (CKC), in standard solution or cationic emulsion formulations in rabbit eyes using newly developed in vivo and ex vivo experimental approaches.
J Hartinger et al.
Analytical biochemistry, 240(1), 126-133 (1996-08-15)
Integral membrane proteins, particularly those with more than one transmembrane domain, have traditionally been difficult to separate by two-dimensional electrophoretic methods. Here we report the adaptation of a previously published procedure [D. E. Macfarlane (1989) Anal. Biochem. 176, 457-463] for
Marco Morciano et al.
Journal of neurochemistry, 95(6), 1732-1745 (2005-11-05)
The nerve terminal proteome governs neurotransmitter release as well as the structural and functional dynamics of the presynaptic compartment. In order to further define specific presynaptic subproteomes we used subcellular fractionation and a monoclonal antibody against the synaptic vesicle protein
Hans Gerd Nothwang et al.
Methods in molecular biology (Clifton, N.J.), 528, 269-277 (2009-01-21)
Defining membrane proteomes is fundamental to our understanding of many physiological and pathophysiological processes. Their separation and identification is hence a key issue in basic and biomedical research. Due to their hydrophobic character, few high-resolution techniques for separation are available
P H R Wilson et al.
International journal of paediatric dentistry, 12(3), 215-218 (2002-05-25)
For many clinicians and parents "teething" remains a convenient diagnosis to explain all manner of local and systemic upset in the young child. Many therapies are on the market to help alleviate the symptoms of primary tooth eruption. In this
Prabhat K Singh et al.
Chemical communications (Cambridge, England), 47(24), 6912-6914 (2011-05-20)
The reactivity between two charged molecules and the activity of charged biomolecules are mainly governed by the principle of electrostatic interaction, i.e., like charges repel and opposite charges attract. In the present study it is shown that the principle of
Zlata Grenoble et al.
The journal of physical chemistry. B, 117(1), 259-272 (2012-12-29)
The adsorption of the cationic surfactant benzyldimethylhexadecylammonium (BDMHA(+)) chloride has been studied at the hydrophilic silica-water interface by Raman spectroscopy in total internal reflection geometry (TIR Raman). This Raman spectroscopic technique takes advantage of an evanescent electric field that is
Yahya Mrestani et al.
International journal of pharmaceutics, 309(1-2), 67-70 (2005-12-27)
The oral application is the application of the first choice for drug administration. A lot of drugs exhibit relatively low bioavailability. This may be caused by binding of the drug in the gastro-intestinal tract, by poor penetration of the intestinal
C Díaz-Nava et al.
Environmental technology, 29(11), 1229-1239 (2008-11-04)
The removal of phenol from aqueous solutions using surfactant-modified clinoptilolite-rich tuffs (SMZ) prepared from two Mexican zeolitic tuffs (Chihuahua and Oaxaca) was investigated. Sodium homoionization of the zeolitic rocks was performed before they were modified with the surfactants: hexadecyltrimethylammonium chloride
Stephan Philipp et al.
Electrophoresis, 33(7), 1120-1128 (2012-04-28)
The intraerythrocytic stage of Plasmodium falciparum alters the characteristics of its host cell by exporting selected plasmodial proteins. Although it is clear that the physicochemical and immunobiological properties of the host cell are modulated during parasite development, the involved plasmodial
Sharmistha Dutta Choudhury et al.
The journal of physical chemistry. B, 113(19), 6736-6744 (2009-04-21)
The prototropic behavior of the dye, 7-hydroxy-4-methylcoumarin (7H4MC), has been studied in cationic benzyldimethylhexadecylammonium chloride (BDHC) and nonionic poly(oxyethylene)(tetramethylbutyl)phenyl ether (TritonX-100, TX-100) reverse micelles using ground-state absorption and steady-state and time-resolved fluorescence measurements. The results have been compared with the
Occupational contact dermatitis to Mikrobac Extra in 8 hospital cleaners.
Layla Haj-Younes et al.
Contact dermatitis, 54(1), 69-70 (2006-01-24)
Cristian C Villa et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 15(14), 3097-3109 (2014-07-22)
The behavior of water entrapped in reverse micelles (RMs) formed by two catanionic ionic liquid-like surfactants, benzyl-n-hexadecyldimethylammonium 1,4-bis-2-ethylhexylsulfosuccinate (AOT-BHD) and cetyltrimethylammonium 1,4-bis-2-ethylhexylsulfosuccinate (AOT-CTA), was investigated by using dynamic (DLS) and static (SLS) light scattering, FTIR, and (1)H NMR spectroscopy techniques.
Daniela Schluesener et al.
Analytical and bioanalytical chemistry, 389(4), 1055-1064 (2007-01-16)
The membrane proteomes of a wild-type Corynebacterium glutamicum and an L-lysine-producing strain were quantitatively analyzed by two complementary proteomics techniques -- anion exchange chromatography AIEC/SDS-PAGE and 16BAC-PAGE/SDS-PAGE -- and the results were compared. Although both techniques allow for the fast
J Schindler et al.
Journal of neural transmission (Vienna, Austria : 1996), 113(8), 995-1013 (2006-07-13)
Subcellular fractionation represents an essential technique for functional proteome analysis. Recently, we provided a subcellular fractionation protocol for minute amounts of tissue that yielded a nuclear fraction, a membrane and organelle fraction, and a cytosolic fraction. In the current study
Hari Kosanam et al.
Analytical chemistry, 79(15), 6020-6026 (2007-07-03)
Detection of doping agents in urine frequently requires extensive separation prior to chemical analyses. Gas or liquid chromatography coupled to mass spectrometry has produced accurate and sensitive assays, but chromatographic separations require time and, sometimes, chemical derivatization. To avoid such
D E Macfarlane
Analytical biochemistry, 176(2), 457-463 (1989-02-01)
A two dimensional preparative polyacrylamide gel electrophoresis system is described which has both high resolving power and high loading capacity (approximately 100 mg protein). The first dimension is electrophoresis toward the cathode at acid pH in the presence of the
Ralf J Braun et al.
Methods in molecular biology (Clifton, N.J.), 528, 83-107 (2009-01-21)
Mitochondria are essential organelles in cellular metabolism. These organelles are bounded by two membranes, the outer and inner membrane. Especially the inner membrane comprises a high content of proteins, for example, the protein complexes of the respiratory chain. High-resolution separation
R C Beier et al.
Journal of applied microbiology, 118(2), 326-342 (2014-11-29)
To evaluate susceptibility of Pseudomonas aeruginosa veterinary isolates to antibiotics and disinfectants. Pseudomonas aeruginosa isolates collected from dogs (n = 155) and other animals (n = 20) from sixteen states during 1994-2003 were tested for susceptibility. Most isolates were resistant
M A Osinski et al.
Journal of the autonomic nervous system, 45(2), 164-174 (1993-11-01)
Mast cell hyperplasia and changes in phenotypic characteristics subsequent to myenteric and extrinsic denervation of a segment of rat jejunum were studied. The myenteric plexus and extrinsic nerves were ablated by serosal application of the cationic surfactant benzyldimethyltetradecylammonium chloride. There
Dara Fitzpatrick et al.
International journal of pharmaceutics, 311(1-2), 139-146 (2006-01-24)
This is the second in a series of papers that report on the release and transport of a range of anionic drugs (diclofenac, salicylic acid) from liquid crystalline gels and ultimately on their use in transdermal delivery. The previous paper
Holly D Coughenour et al.
Proteomics, 4(10), 3141-3155 (2004-09-21)
A proteomic analysis of the synaptic vesicle was undertaken to obtain a better understanding of vesicle regulation. Synaptic vesicles primarily consist of integral membrane proteins that are not well resolved on traditional isoelectric focusing/two-dimensional gel electrophoresis (IEF/2-DE) gels and are
D E Macfarlane
Analytical biochemistry, 132(2), 231-235 (1983-07-15)
A discontinuous polyacrylamide gel system operating at pH 4.0-1.5 which resolves proteins bearing base labile groups extracted from intact cells is described. It uses potassium phosphate buffer in the running and stacking gel and glycine as the trailing ion component.
Jacqueline Burré et al.
Electrophoresis, 27(17), 3488-3496 (2006-09-01)
Synaptic vesicles are organelles of the nerve terminal that secrete neurotransmitters by fusion with the presynaptic plasma membrane. Vesicle fusion is tightly controlled by depolarization of the plasma membrane and a set of proteins that may undergo post-translational modifications such
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