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3D cell culture
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Showing 1-22 of 22 results
Cancer Stem Cell Tumorsphere Formation Protocol
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
Corning® Spheroid Microplates
Understand how Corning® Spheroid Microplates can be used for generating and culturing tumor spheroids in a 96-well spheroid microplate format.
Protocol Guide: Immunofluorescent Staining of Whole-Mount Organoids using Antibodies
How to stain organoids? A complete step-by-step protocol for immunofluorescent (IF) and immunocytochemical (ICC) staining of organoid cultures using antibodies
Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro
Learn how the Corning® Matrigel® Basement Membrane Matrix can be used to boost your 3D Cell Culture activities.
TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) preparation protocol
A specific protocol for TrueGel3D™ hydrogel CD cell-degradable crosslinker and RGD peptide (TRUE1) kits for 3D cell culture is available
Generating Organotypic Air-Liquid Interface (ALI) 3D Skin Cultures as an In-Vitro Model of the Human Epidermis
3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.
Laminin Coating Protocol for Cell Culture
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Human Neural Stem Cells Differentiation with TrueGel3D®
Cultivate ReNcell® human neural stem cells in 3D hydrogels for high-throughput screening using the TrueGel3D® HTS Hydrogel Plate with this protocol.
TrueGel3D™ Hydrogel Prep Protocol
TrueGel3D™ hydrogel enables cell encapsulation for 3D culture, mimicking native tissue environment with chemically defined properties.
Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol
3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.
Cultrex® 3D Spheroid BME Invasion Assay Protocol
3D Spheroid BME Cell Invasion Assay utilizes specialized plates and ECM for cell aggregation and spheroid formation in vitro.
TrueGel3D™ Hydrogel Prep Protocol
TrueGel3D™ is a chemically defined hydrogel for 3D cell growth, facilitating microchannel filling with slow gelation.
Cultrex® 3-D Culture Matrix™ Laminin I Protocol
3-D Culture Matrix™ Mouse Laminin I may be used as a gel or as a media additive to study cellular growth and differentiation in three dimensions in vitro.
In Vitro Differentiation of Human iPS Cells Into Colon Organoids in Serum-Free Cell Culture Conditions
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells.
Advanced Protocol: How to Customize your TrueGel3D™ Hydrogel
An advanced protocol is available for TrueGel 3D kits for 3D cell culture for peptides concentration or stiffness optimisation
Tumor Spheroid Formation Assay
3D cell culture protocol: the tumor spheroid formation assay using a serum-free and xeno-free cancer stem cell culture media.
Poly-L-Lysine Cell Attachment Protocol
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration
Learn the results that come from changing the protein concentration of Corning® Matrigel®.
Organoids Generation and Phenotypic Screen on Millicell® Microwell Plates
Discover how to use Millicell® Microwell plates to generate mouse intestinal and colorectal cancer organoids and develop a high throughput phenotypic drug development screen.
T-Cell Migration Assays Using Millicell® Cell Culture Inserts
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
Guide: Expansion & Differentiation of Human Mesenchymal
TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.
In Vitro T Cell Killing Assay Protocol
Millicell® Microwell plates facilitate T cell killing assays, evaluating killing capacity in tumor spheroids for personalized assays.